HSP60和NRF2在透明细胞性肾细胞癌中的表达及临床价值  

Expression and clinical value of HSP60 and NRF2 in clear cell renal cell carcinoma

作　　者：鲍晨[1] 董学敏[1] 杨静茹 胡倩倩 柳俊[1] 米琳[2] 王勇 BAO Chen;DONG Xuemin;YANG Jingru;HU Qianqian;LIU Jun;MI Lin;WANG Yong(Department of Pathology,Ankang Central Hospital,Ankang,Shaanxi 725000,China;Department of Nephrology,Ankang Central Hospital,Ankang,Shaanxi 725000,China;Department of Nephrology,Shaanxi Provincial People′s Hospital,Xi′an,Shaanxi 710068,China)

机构地区：[1]安康市中心医院病理科,陕西安康725000 [2]安康市中心医院肾内科,陕西安康725000 [3]陕西省人民医院肾内科,陕西西安710068

出　　处：《国际检验医学杂志》2022年第5期574-579,共6页International Journal of Laboratory Medicine

基　　金：陕西省科学技术研究发展计划项目(2017K15-02-794)。

摘　　要：目的研究透明细胞性肾细胞癌(ccRCC)中热休克蛋白60(HSP60)及核因子E2相关因子2(NRF2)的表达及临床意义。方法选取2017年3月至2019年3月在安康市中心医院诊治的89例ccRCC患者为研究对象,应用荧光定量聚合酶链反应(qPCR)及免疫组织化学检测癌组织及癌旁组织中HSP60、NRF2的表达。统计分析ccRCC中HSP60、NRF2的表达与临床病理特征关系。采用Spearmen秩相关分析HSP60与NRF2 mRNA表达的相关性,Kaplan-Meier生存分析(Log-Rank检验)ccRCC中HSP60、NRF2的表达与患者生存预后的关系,单因素和多因素COX比例风险模型分析影响ccRCC患者生存预后的危险因素。结果ccRCC癌组织HSP60的相对表达量(0.483±0.112)明显低于癌旁组织(1.227±0.265),差异有统计学意义(t=24.397,P<0.001),而NRF2 mRNA的相对表达量(1.725±0.285)明显高于癌旁组织(0.551±0.101),差异有统计学意义(t=36.629,P<0.001)。ccRCC癌组织中HSP60与NRF2的表达呈明显负相关(r=-0.520,P<0.001)。ccRCC癌组织中HSP60、NRF2的表达与肿瘤分期、远处转移及淋巴结转移有关(χ^(2)=11.749、24.815、45.762,17.225、21.913、7.061,均P<0.05)。HSP60低表达组3年生存率为41.5%(17/41),明显高于HSP60高表达组的3年生存率93.2%(41/44),差异有统计学意义(χ^(2)=30.79,P<0.001);HSP60低表达组平均生存时间为(23.5±6.1)个月,明显高于HSP60高表达组的平均生存时间(28.2±6.4)个月,差异有统计学意义(t=3.460,P<0.001)。NRF2低表达组3年生存率为95.2%(40/42),明显高于NRF2高表达组的3年生存率41.9%(18/43),差异有统计学意义(χ^(2)=30.79,P<0.001),HSP60低表达组平均生存时间为(29.1±6.2)个月,明显高于HSP60高表达组的平均生存时间(22.4±6.3)个月,差异有统计学意义(χ^(2)=4.941,P<0.001)。多因素Cox回归分析结果显示,ccRCC组织中HSP60低表达、NRF2高表达、肿瘤分期Ⅲ~Ⅳ期是影响ccRCC患者不良预后的独立危险因素。结论ccRCC中HSP60表达降低,而NRF2表达升高,�Objective To study the expression of heat shock protein 60(HSP60)and nuclear factor E2 related factor 2(NRF2)in clear cell renal cell carcinoma(ccRCC)and their clinical significance.Methods The clinical data of 89 ccRCC patients diagnosed and treated in our hospital from March 2017 to March 2019 were selected.Fluorescence quantitative PCR and immunohistochemistry were used to detect the expression of HSP60 and NRF2 in cancer and adjacent tissues.The relationship between the expression of HSP60,NRF2 and clinicopathological characteristics in ccRCC was statistically analyzed.The correlation between HSP60 and NRF2 mRNA expression levels were analyzed by Spearmen rank correlation analysis.The correlation between the expression of HSP60,NRF2 with prognosis of ccRCC patients were analyzed by Kaplan-Meier survival analysis(Log-Rank test).Univariate and multivariate COX proportional hazard models were used to analyze the risk factors that affected the survival and prognosis of renal cancer patients.Results The relative expression of HSP60 in ccRCC cancer tissue(0.483±0.112)was significantly lower than that of adjacent tissues(1.227±0.265),and the difference was statistically significant(t=24.397,P<0.001),while the relative expression of NRF2 mRNA(1.725±0.285)in ccRCC cancer tissue was significantly higher than that of adjacent tissues(0.551±0.101),and the difference was statistically significant(t=36.629,P<0.001).The expression levels of HSP60 and NRF2 in ccRCC cancer tissue were significantly negatively correlated(r=-0.520,P<0.001).The expression levels of HSP60 and NRF2 in ccRCC cancer tissues were related to tumor stage,distant metastasis and lymph node metastasis and the differences were statistically significant(χ^(2)=11.749,24.815,45.762,17.225,21.913,7.061,all P<0.05).The 3-year survival rate was 41.5%(17/41)in HSP60 low expression group,which was significantly higher than that in high expression group of 93.2%(41/44),and the difference was statistically significant(χ^(2)=30.79,P<0.001);The average survival

关 键 词：透明细胞性肾细胞癌 热休克蛋白60 核因子E2相关因子2 临床意义 

分 类 号：R737.11[医药卫生—肿瘤]