高效液相色谱-质谱联用法测定姜黄素在大鼠体内的分布  

Analysis the absorption of curcumin in rat by LC-MS/MS

作　　者：程继[1] 赵华才[1] 王志刚[1] 曹建伟[1] 马浩鑫 董青川[1] CHENG Ji;ZHAO Huacai;WANG Zhigang;CAO Jianwei;MA Haoxin;DONG Qingchuan(Department of Urology,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China)

机构地区：[1]陕西省人民医院泌尿外科,陕西西安710068

出　　处：《现代肿瘤医学》2022年第6期991-995,共5页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划一般项目(编号:2019JM-069)。

摘　　要：目的:采用高效液相色谱-质谱联用法,测定姜黄素在大鼠血浆和组织中药物浓度随时间变化的分布规律及药物代谢动力学的特点。方法:大鼠口服灌胃姜黄素后,收集血浆和组织样品,通过有机溶剂乙酸乙酯分离提取,采用高效液相色谱-质谱联用法分析,选择C18色谱分析柱,乙腈(85%)和水(5%)为流动相,检测血浆和组织中的姜黄素药物浓度。质谱条件为电喷雾电离正离子方式(ESI+),多反应监测(MRM)模式。结果:姜黄素在血中的达峰时间为0.5 h,曲线下面积(AUC)为(236±6)ng·h/mL,峰值血药浓度为(91±5)ng/mL;心脏中的达峰时间为1 h,肝脏和肾脏中的达峰时间均为2 h。在心脏和肝脏中,曲线下面积(AUC)分别为(127±3)ng·h/mL和(166±5)ng·h/mL,均显著高于其他组织。结论:我们建立的高效液相色谱-质谱联用分析方法,可有效监测姜黄素在血中或组织器官中药物浓度随时间变化的规律,此方法简便、重复性高、灵敏度强。大鼠口服姜黄素后,可被吸收入血,组织器官分布广泛。Objective:To determine the distribution of curcumin and the characteristics of pharmacokinetics by high performance liquid chromatography-mass spectrometry(LC-MS/MS)in rat plasma and in vivo.Methods:Curcumin samples in rat plasma or tissue were collected and extracted by organic solvent ethyl acetate after oral administration.The method of high performance liquid chromatography-mass spectrometry with C18 chromatography column including acetonitrile(85%)and water(5%)as the mobile phase was used to analyzing the curcumin concentration in rat plasma or tissue.The mass spectrometry conditions were selected with electrospray ionization positive ion mode(ESI+)and multiple reaction monitoring(MRM)mode.Results:The peak time of curcumin was 0.5 in rat plasma and the AUC area was(236±6)ng·h/mL.In addition,the highest concentration of curcumin was(91±5)ng/mL in rat plasma.The peak time of curcumin was 1 in rat heart,but it was 2 in liver or kidneys,respectively.And the AUC area were(127±3)ng·h/mL and(166±5)ng·h/mL in the heart and liver,respectively.Conclusion:This LC-MS/MS method will be used to observe the characteristics of pharmacokinetics of curcumin rat plasma effectively.It was simple,highly reproducible and sensitive method.Curcumin could be absorbed into the blood,and distributed to the tissues widely after oral administration.

关 键 词：姜黄素 高效液相色谱-质谱联用 生物利用度 药代动力学 分布 

分 类 号：R730.5[医药卫生—肿瘤]