HIF-1α、COX-2、CRP及ESR在溃疡性结肠炎患者外周血及黏膜组织中的表达及临床意义  

Expression and clinical significance of HIF-1α,COX-2,CRP and ESR in peripheral blood and mucosal tissues of patients with ulcerative colitis

作　　者：苏淑伶 苏龙 郑丽丽 张艳艳[2] SU Shu-ling;SU Long;ZHENG Li-li(Department of Laboratory Medicine,Shunyi Hospital,Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China)

机构地区：[1]北京中医医院顺义医院检验科,北京101300 [2]首都医科大学附属北京安贞医院检验科,北京100029

出　　处：《临床和实验医学杂志》2022年第4期415-419,共5页Journal of Clinical and Experimental Medicine

基　　金：北京市中医药科技项目(编号:JJ2018-39)。

摘　　要：目的研究溃疡性结肠炎(UC)患者外周血和黏膜组织中缺氧诱导因子1α(HIF-1α)、环氧酶-2(COX-2)、C-反应蛋白(CRP)和红细胞沉降率(ESR)表达水平,并探讨其临床意义。方法回顾性选取2018年1月至2020年12月在北京中医医院顺义医院治疗的UC患者120例作为研究对象,根据Walmsley评分分为缓解期40例(Walmsley评分<4分)和活动期80例(Walmsley评分>4分)。并选择同期结肠镜检测健康患者15例作为对照组。比较3组患者外周血HIF-1α、COX-2、CRP及ESR水平,以及黏膜组织HIF-1α、COX-2、CRP的表达水平。结果与对照组相比,UC患者血清HIF-1α、COX-2、CRP含量以及外周血ESP显著升高,差异均有统计学意义(P<0.05);活动期UC患者血清HIF-1α、COX-2、CRP含量以及外周血ESP显著高于缓解期UC患者,差异均有统计学意义(P<0.05)。与对照组相比,UC患者黏膜组织HIF-1α、COX-2和CRP mRNA和蛋白表达水平显著升高,差异均有统计学意义(P<0.05);活动期UC患者黏膜组织HIF-1α、COX-2和CRP mRNA和蛋白表达水平显著高于缓解期UC患者,差异均有统计学意义(P<0.05)。结论HIF-1α、COX-2、CRP及ESR不仅与UC的发病有关,而且与UC疾病进展有关。Objective To study the expression of hypoxia inducible factor 1α(Hypoxia inducible factor 1α),cyclooxygenase-2(COX-2),and C-reactive protein C-reactive protein(CRP)and erythrocyte sedimentation rate(ESR)in peripheral blood and mucosal tissues of patients with ulcerative colitis(UC),and explore their clinical significance.Methods A total of 120 UC patients treated in Shunyi Hospital,Beijing Hospital of Traditional Chinese Medicine from January 2018 to December 2020 were retrospectively selected,and divided into 40 cases in remission period(Walmsley score<4 points)and 80 cases in active period(Walmsley score>4 points)according to Walmsley score.And 15 healthy patients were selected as the control group during the same period of colonoscopy.The levels of HIF-1α,COX-2,CRP,and ESR,and the expression levels of HIF-1α,COX-2,and CRP in the peripheral blood and mucosal tissues of the three groups were compared.Results Compared with the control group,the levels of serum HIF-1α,COX-2,CRP and peripheral blood ESP in UC patients were significantly increased,the differences were statistically significant(P<0.05);the levels of serum HIF-1α,COX-2,CRP and peripheral blood ESP in active UC patients was significantly higher than those in remission period UC patients,the differences were statistically significant(P<0.05).Compared with the control group,the expression levels of HIF-1α,COX-2 and CRP mRNA and protein in the mucosal tissues of UC patients were significantly increased,and the differences were statistically significant(P<0.05);the expression levels of HIF-1α,COX-2 and CRP mRNA and protein in the mucosal tissues in active UC patients were significantly higher than those in remission period UC patients,and the differences were statistically significant(P<0.05).Conclusion HIF-1α,COX-2,CRP and ESR are not only related to the pathogenesis of UC,but also related to the progression of UC disease.

关 键 词：溃疡性结肠炎 缺氧诱导因子1Α 环氧酶-2 C-反应蛋白 

分 类 号：R574.62[医药卫生—消化系统]