宫颈癌PIK3CA蛋白阳性表达率与病理特征、抗肿瘤免疫应答的相关性分析  

Correlation analysis of PIK3CA protein positive expression rate with pathological characteristics and anti-tumor immune response in cervical cancer

作　　者：陈春妃 周小飞 方秋满 邓青春 CHEN Chun-fei;ZHOU Xiao-fei;FANG Qiu-man(Department of Gynaecology,the Second Affiliated Hospital of Hainan Medical University,Haikou Hainan 570311,China)

机构地区：[1]海南医学院第二附属医院妇科,海南海口570311

出　　处：《临床和实验医学杂志》2022年第4期419-422,共4页Journal of Clinical and Experimental Medicine

基　　金：2020年海南省科学技术协会青年科技英才创新计划项目(编号:QCXM202018);2020年海南省卫健委项目(编号:20A200247)。

摘　　要：目的研究宫颈癌磷脂酰肌醇激酶-3催化亚单位α(PIK3CA)蛋白阳性表达率与病理特征、抗肿瘤免疫应答的相关性。方法回顾性选择2017年1月至2018年3月在海南医学院第二附属医院接受根治性手术的108例宫颈癌患者作为宫颈癌组,并留取宫颈癌组织;同期因子宫肌瘤接受手术切除的68例患者作为对照组,并留取正常宫颈组织。采用免疫组织化学染色检测宫颈癌组织及正常宫颈组织中PIK3CA蛋白阳性表达率。采用酶联免疫吸附试验检测宫颈癌患者术前血清中免疫细胞因子白细胞介素(IL)-2、IL-4、IL-5、IL-10、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)的含量。收集宫颈癌患者的病理特征,随访宫颈癌患者的无病生存情况及总生存情况。结果宫颈癌组织中PIK3CA的蛋白阳性表达率为77.78%,高于正常宫颈组织(50.00%),差异有统计学意义(P<0.05)。宫颈癌组织PIK3CA蛋白阳性患者中FIGO分期ⅡA期、有脉管浸润、有淋巴结转移的比率为78.57%、48.81%、40.48%,高于PIK3CA蛋白阴性患者(54.17%、20.83%、16.67%),差异均有统计学意义(P<0.05),病理类型、分化程度与PIK3CA蛋白阴性患者比较差异无统计学意义(P>0.05)。宫颈癌组织PIK3CA蛋白阳性患者的血清IL-2、TNF-α、IFN-γ含量为(3.04±0.89)pg/mL、(10.61±1.83)ng/mL、(13.24±2.92)ng/mL,低于PIK3CA蛋白阴性患者[(4.13±1.14)pg/mL、(13.84±4.52)ng/mL、(17.02±4.95)ng/mL],血清IL-4、IL-5、IL-10含量为(2.98±0.52)、(4.42±1.09)、(6.76±1.14)pg/mL,高于PIK3CA蛋白阴性表达患者[(2.24±0.77)、(3.18±0.71)、(5.14±0.93)pg/mL],差异均有统计学意义(P<0.05)。宫颈癌组织PIK3CA蛋白阳性患者随访过程中的累积无病生存率及累积总生存率均低于PIK3CA蛋白阴性表达患者,差异有统计学意义(P<0.05)。结论宫颈癌中PIK3CA表达增加与病理进展、抗肿瘤免疫应答减弱及远期生存率降低有关。Objective To study the correlation of phosphatidylinositol 3-kinase catalytic alpha polypeptide(PIK3CA)protein positive expression rate with pathological characteristics and anti-tumor immune response in cervical cancer.Methods A total of 108 patients with cervical cancer who underwent radical surgery in the Second Affiliated Hospital of Hainan Medical University from January 2017 to March 2018 were retrospectively selected as the cervical cancer group and cervical cancer tissues were collected,68 patients who underwent surgical resection due to uterine fibroids at the same time were selected as the control group and normal cervical tissues were selected.The positive expression rate of PIK3CA protein in cervical cancer tissues and normal cervical tissues was detected by immunohistochemical staining,the serum contents of immune cytokines interleukin(IL)-2,IL-4,IL-5,IL-10,tumor necrosis factor-α(TNF-α),Interferon-γ(IFN-γ)were detected by ELISA.The pathological characteristics of patients with cervical cancer were collected,disease-free survival and overall survival of patients with cervical cancer were followed-up.Results The positive expression rate of PIK3CA protein in cervical cancer tissue was 77.78%,which was higher than that in normal cervical tissue(50.00%),and the difference was statistically significant(P<0.05).The proportion of FIGO stage IIA,vascular invasion,and lymph node metastasis in cervical cancer tissue positive patients with PIK3CA protein were 78.57%,48.81%,and 40.48%,which were higher than those in PIK3CA protein-negative patients(54.17%,20.83%,16.67%),the differences were statistically significant(P<0.05),and there was no significant difference in pathological type,differentiation degree and PIK3CA protein-negative patients(P>0.05).The serum levels of IL-2,TNF-αand IFN-γin patients with positive PIK3CA protein in cervical cancer tissue were(3.04±0.89)pg/mL,(10.61±1.83)ng/mL,(13.24±2.92)ng/mL,which were lower than those in patients with negative PIK3CA protein[(4.13±1.14)pg/mL,(13.84�

关 键 词：宫颈癌 磷脂酰肌醇激酶-3催化亚单位α 病理特征 抗肿瘤免疫应答 预后 

分 类 号：R737.33[医药卫生—肿瘤]