融合蛋白mCry1AbVip3A对亚洲玉米螟和草地贪夜蛾的抗虫效率分析  

Analysis of Insect Resistance Efficiency of the Fusion Protein mCry1AbVip3A against Ostrinia furnacalis and Spodoptera frugiperda

作　　者：李望舒 武奉慈[3] 宋新元[3] 王振华[1] 翁建峰[2] 李新海[2] LI Wang-shu;WU Feng-chi;SONG Xin-yuan;WANG Zhen-hua;WENG Jian-feng;LI Xin-Hai(Agricultural College,Northeast Agricultural University,Harbin 150030;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]中国农业科学院作物科学研究所,北京100081 [3]吉林省农业科学院,长春130033

出　　处：《玉米科学》2022年第1期69-76,共8页Journal of Maize Sciences

基　　金：抗草地贪夜蛾兼耐热玉米新品种培育及市场推广(SKJC-2020-02-005)。

摘　　要：将融合基因mCry1AbVip3A构建原核表达载体pET30A-mCry1AbVip3A,转入大肠杆菌BL21(DE3),通过优化诱导条件获得mCry1AbVip3A蛋白。室内生测mCry1AbVip3A蛋白对亚洲玉米螟和草地贪夜蛾的杀虫效果,发现饲喂终浓度为128.44 ng/mL的mCry1AbVip3A蛋白的亚洲玉米螟1龄幼虫和草地贪夜蛾1龄、2龄幼虫存活率均为0;草地贪夜蛾3龄和4龄幼虫的存活率分别为23.33%和36.67%。结果表明,mCry1AbVip3A蛋白对亚洲玉米螟1龄幼虫的杀虫效果与Cry1Ab-ma蛋白无显著差异,显著高于Vip3A蛋白;mCry1AbVip3A蛋白对草地贪夜蛾3龄和4龄幼虫的杀虫效果与Vip3A蛋白无显著差异,显著高于Cry1Ab-ma蛋白。构建植物过表达载体pTF101.1-mCry1AbVip3A进行玉米遗传转化并获得转基因事件,为转基因抗亚洲玉米螟和草地贪夜蛾玉米育种提供了新材料。The prokaryotic expression vector pET30 A-mCry1 AbVip3 A was constructed and transferred into E.coli BL21(DE3)for induction and expression,and mCry1 AbVip3 A protein was obtained by optimizing induction conditions.Lab bioassay of insecticidal effect of mCry1 AbVip3 A protein on Ostrinia furnacalis and Spodoptera frugiperda was performed.The final concentration of 128.44 ng/mL of mCry1 AbVip3 A protein was fed to Ostrinia furnacalis and Spodoptera frugiperda.The result showed that the survival rates of the 1 st instar larvae of Ostrinia furnacalis,and the 1 st and 2 nd instar larvae of Spodoptera frugiperda were 0;the survival rates of the 3 rd and 4 th instar larvae of Spodoptera frugiperda were 23.33%and 36.67%,respectively.Comparative analysis showed that the insecticidal effect of mCry1 AbVip3 A protein and Cry1 Ab-ma protein on the first instar larvae of Ostrinia furnacalis was not significantly different,but significantly higher than the Vip3 A protein;the insecticidal effect of mCry1 AbVip3 A on the 3 rd and 4 th instar larvae of Spodoptera frugiperda was not significantly different from that of Vip3 A protein but was significantly higher than that of Cry1 Ab-ma protein.The plant overexpression vector pTF101.1-mCry1 AbVip3 A was constructed for maize genetic transformation and transgenic events were obtained,providing new materials for transgenic corn breeding resistant to Ostrinia furnacalis and Spodoptera frugiperda.

关 键 词：玉米 融合基因 原核表达 抗虫效率 遗传转化 

分 类 号：S513.035.3[农业科学—作物学]