上调miR-145表达抑制卵巢癌HO8910细胞侵袭、迁移及促进TβR-Ⅱ蛋白表达  

Up-regulation of miR-145 expression inhibits invasion and migration of ovarian cancer HO8910 cells and promotes TβR-Ⅱ protein expression

作　　者：祁振军 戴志琴[2] QI Zhenjun;DAI Zhiqin(Department of Laboratory,Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research&the Affiliated Cancer Hospital of Nanjing Medical University,Jiangsu Nanjing 210009,China;Department of Gynecology and Oncology,Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research&the Affiliated Cancer Hospital of Nanjing Medical University,Jiangsu Nanjing 210009,China)

机构地区：[1]江苏省肿瘤医院/江苏省肿瘤防治研究所/南京医科大学附属肿瘤医院检验科,江苏南京210009 [2]江苏省肿瘤医院/江苏省肿瘤防治研究所/南京医科大学附属肿瘤医院妇瘤科,江苏南京210009

出　　处：《现代肿瘤医学》2022年第5期779-784,共6页Journal of Modern Oncology

摘　　要：目的:探究微小RNA-145(microRNA-145,miR-145)对卵巢癌(ovarian cancer, OC)HO8910细胞侵袭、迁移及TβR-Ⅱ蛋白水平的影响。方法:将某大学研究实验室细胞库提供的OC细胞HO8910分为ZW组(无转染HO8910细胞)、ZN组(转染miR-145-NC)、YJ组(转染miR-145 mimics)。采用qRT-PCR、MTT、Hoechst33258荧光染色、Transwell小室、细胞划痕、Western blot检测miR-145、转化生长因子βⅡ型受体(transforming growth factorβtypeⅡreceptor, TβR-Ⅱ)表达、细胞活力、凋亡、侵袭、迁移能力以及TβR-Ⅱ蛋白量。结果:YJ组HO8910细胞中miR-145的表达量最高,提示转染成功。YJ组TβR-Ⅱ表达量较其他两组对比明显提高(P<0.05),ZW组、ZN组miR-145、TβR-ⅡmRNA表达水平无明显差异(P>0.05);培养48、72、96 h, YJ组细胞活力较ZW组、ZN组比较明显减弱(P<0.05),随着时间的延续,培养96 h三组细胞活力均优于48、72 h(P<0.05);YJ组与ZW组、ZN组对比细胞凋亡率明显提高(P<0.05);ZW组HO8910细胞荧光强度最弱,细胞凋亡率最低,ZW组与ZN组对比无明显差异(P>0.05);三组细胞侵袭、迁移数量相比差异较大(P<0.05),其中YJ组侵袭、迁移数量少于其他两组(均P<0.05),ZN组与ZW组的细胞侵袭、迁移数量无明显差异(P> 0.05);YJ组TβR-Ⅱ蛋白表达量与ZU组、ZN组对比明显升高(P<0.05),YJ组转化生长因子-β(transforming growth factorβ,TGF-β)蛋白表达量与其他两组对比均降低(P<0.05),ZW组TGF-β、TβR-Ⅱ蛋白表达量与ZN组对比差异较小(P> 0.05)。结论:通过上调miR-145对抑制OC细胞迁移、侵袭等生物活性发挥积极作用,分析原因可能与靶向调控TβR-Ⅱ蛋白相关。Objective:To understand the effect of miR-145 on the invasion and migration of OC HO8910 cells and the changes of TβR-Ⅱ protein.Methods:OC HO9810 cells provided by one laboratory cell bank were divided into ZW group(HO8910 cells),ZN group(miR-145-NC),and YJ group(miR-145 mimics).qRT-PCR,MTT,Hoechst33258 fluorescence staining, Transwell cell, cell scratches, Western blot detected miR-145,TβR-Ⅱ expression, cell viability, apoptosis, invasion, migration ability and TβR-Ⅱ protein.Results:The expression level of miR-145 in HO8910 cells in the YJ group was the highest, indicating successful transfection.The expression of TβR-Ⅱ in the YJ group was different from that of the other two groups(P<0.05).The mRNA expression levels of miR-145 and TβR-Ⅱ in the ZW group and ZN group were the same(P>0.05).After 48,72,and 96 hours of culture, the activity of HO8910 cells in the YJ group was the weakest compared to the ZW group and the ZN group(P<0.05).With the continuation of time, the cell viability of the three groups after 96 h culture was better than 48 and 72 h(P<0.05).The apoptosis rate of HO8910 cells in the YJ group was higher than that in the ZW group and ZN group(P<0.05).The HO8910 cells in the ZW group had the weakest fluorescence intensity and the lowest apoptosis rate.The ZW group was the same as the ZN group(P>0.05).The number of cell invasion and migration in the three groups was quite different(P<0.05).The number of invasions and migrations in the YJ group was less than the other two groups(all P<0.05).There was no significant difference in the number of cell invasion and migration between ZN group and ZW group(P>0.05).The expression of TβR-Ⅱ protein in the YJ group was significantly higher than that in the ZW group and ZN group(P<0.05).The expression of TGF-β protein in the YJ group was reduced compared with the other two groups(P<0.05),and the expression levels of TGF-β and TβR-Ⅱ proteins in ZW group were less different from those of ZN group(P>0.05).Conclusion:Up-regulating miR-145 can pla

关 键 词：OC MIR-145 TβR-Ⅱ HO8910细胞 

分 类 号：R737.31[医药卫生—肿瘤]