SNHG1在胆管癌组织及细胞中的表达及意义  

Expression and significance of SNHG1 in cholangiocarcinoma tissues and cells

作　　者：桑海泉[1] 任伟强 陈鹏 SANG Haiquan;REN Weiqiang;CHEN Peng(Department of General Surgery,the Fourth Affiliated Hospital of China Medical University,Liaoning Shenyang 110032,China)

机构地区：[1]中国医科大学附属第四医院普通外科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2022年第5期856-860,共5页Journal of Modern Oncology

基　　金：辽宁省科学技术计划项目(编号:201501018/2020-MS-12)。

摘　　要：目的:探讨长链非编码RNA核仁小分子RNA宿主基因1(small nucleolar RNA host gene 1,SNHG1)在胆管癌组织和细胞中的表达及临床意义。方法:RT-PCR检测64例胆管癌组织及其配对的癌旁组织、胆管癌细胞(RBE和QBC939)和正常肝内胆管上皮细胞(HIBE)中SNHG1的相对表达水平。分析SNHG1与胆管癌临床病理特征的关系,COX多因素分析和Kaplan-Meier生存曲线分析SNHG1与胆管癌患者生存预后的关系。LipofectAMINE;2000将SNHG1过表达质粒(过表达组)、SNHG1敲降质粒(沉默组)和阴性对照质粒(对照组)转入RBE细胞,CCK8法检测细胞增殖;流式细胞仪检测细胞周期;Transwell检测细胞侵袭。结果:癌组织中SNHG1相对表达量明显高于癌旁组织(P<0.05),与HIBE细胞比较,RBE和QBC939细胞的SNHG1相对表达量较高(P均<0.05)。SNHG1与TNM分期和淋巴结转移有关(P均<0.05),而与性别、年龄、肿瘤部位和分化程度无关(P均>0.05)。COX多因素分析结果显示,TNM分期、淋巴结转移和SNHG1高表达是胆管癌患者不良生存预后的独立影响因素(P均<0.05)。Kaplan-Meier生存曲线显示,SNHG1高表达组中位生存时间(17个月)低于低表达组(42个月),差异有统计学意义(χ^(2)=6.698,P=0.003)。与对照组比较,沉默组SNHG1相对表达量,细胞增殖和侵袭活力明显降低(P均<0.05),细胞周期停滞在G1期;而过表达组上述指标明显增高(P均<0.05),细胞周期更多进入G2期。结论:SNHG1可能参与胆管癌的发生和发展,并且与生存预后有关。Objective:To explore the expression and clinical significance of long non-coding RNA nucleolar small molecule RNA host gene 1(SNHG1) in cholangiocarcinoma.Methods:RT-PCR was used to detect the relative expression levels of SNHG1 in 64 cases of cholangiocarcinoma tissues and their paired adjacent tissues, cholangiocarcinoma cells(RBE and QBC939) and normal intrahepaticbile duct epithelial cells(HIBE).The relationship between SNHG1 and the clinicopathological characteristics of cholangiocarcinoma was analyzed, and the relationship between SNHG1 and the survival and prognosis of patients with cholangiocarcinoma was analyzed by COX multivariate analysis and Kaplan-Meier survival curve.SNHG1 overexpression plasmid(overexpression group),SNHG1 knockdown plasmid(silence group) and negative control plasmid(control group) were transferred into RBE cells with LipofectAMINE;2000.Cell proliferation was detected by CCK8 method.Cell cycle was detected by flow cytometry.Cell invasion was detected by Transwell.Results:The relative expression of SNHG1 in cancer tissues was significantly higher than that in adjacent tissues(P<0.05).Compared with HIBE cells, the relative expression of SNHG1 in RBE and QBC939 cells washigher(P<0.05).SNHG1 was related to TNM staging and lymph node metastasis(P<0.05),but not related to gender, age, tumor location and degree of differentiation(P>0.05).COX multivariate analysis showed that TNM staging, lymph node metastasis and high expression of SNHG1 were independent factors influencing the poor survival and prognosis of patients with cholangiocarcinoma(P<0.05).The Kaplan-Meier survival curve showed that the median survival time of high expression group(17 months) was lower than that of low expression group(42 months),and the difference was statistically significant(χ^(2)=6.698,P=0.003).Compared with control group, the relative expression of SNHG1,cell proliferation and invasion activity insilent group were significantly reduced(P<0.05),cell cycle was arrested in G;phase.While the above indicators in

关 键 词：长链非编码RNA 核仁小分子RNA宿主基因1 胆管癌 预后 增殖 细胞周期 侵袭 

分 类 号：R735[医药卫生—肿瘤]