吉林省内花生衣药材中黄曲霉毒素B1、B2、G1、G2的测定  

Determination of Aflatoxin B1,B2,G1 and G2 in Testa Arachis by HPLC-Post-Colum Photochemical Derivatization

作　　者：马晓静[1] 胡洋 李正刚 王丹彧 MA Xiaojing;HU Yang;LI Zhenggang;WANG Danyu(Siping City Institute for Food and Drug Control,Siping Jilin,136000,China;No.964 Hospital Second Outpatient Department)

机构地区：[1]四平市食品药品检验所,吉林四平136000 [2]第964医院第二门诊部

出　　处：《质量安全与检验检测》2022年第1期17-19,共3页QUALITY SAFETY INSPECTION AND TESTING

基　　金：吉林省中药材地方标准制修订项目(JLZYC-2018-007)。

摘　　要：为测定吉林省内花生衣药材中黄曲霉毒素B1、B2、G1、G2的含量,评估样品污染情况,本文采用免疫亲和柱净化、高效液相色谱光化学衍生荧光检测法,以Agilent ZORBAX SB-C18为色谱柱(4.6×250 mm 5μm);以甲醇-乙腈-水(40∶20∶40)为流动相,流速为0.8 mL/min;柱温为25℃;荧光检测器激发波长λex=360 nm,发射波长λex=450 nm。结果显示,AFB1、AFB2、AFG1、AFG2线性关系良好;AFB1、AFB2、AFG1、AFG2检出限依次为0.155μg/kg、0.050μg/kg、0.155μg/kg、0.058μg/kg;定量限依次为0.465μg/kg、0.150μg/kg、0.465μg/kg、0.175μg/kg。由此可见,该方法快速、准确、重复性好,能够为花生衣中黄曲霉毒素测定方法提供科学依据。In order to determine the contents of aflatoxin B1,B2,G1 and G2 in peanut coating medicinal materials in Jilin Province and evaluate the pollution of samples,immunoaffinity column purification and high performance liquid chromatography photochemical derivatization fluorescence detection were used,the colum was Agilent ZORBAX SB-C18 chromatographic column(4.6×250mm,5μm);The mobile phase consisted of methanol-acetomitrile-water(40:20:40);the flow velocity was 0.8ml,Column temperature was 25℃.Excitiation wavelength and emission wavelength of fluorescence detector were 360 nm and 450 nm.The results show that AFB1,AFB2,AFG1,AFG2 showed good linearity.The detection limit of AFB1,AFB2,AFG1 and AFG2 were 0.155μg/kg,0.05μg/kg,0.155μg/kg,0.058μg/kg.The quantitative limits were 0.465μg/kg,0.150μg/kg,0.465μg/kg and 0.175μg/kg,.Thus it can be seen,the method is fast,accurate and reproducible,which provides a scientific basis for the determination of aflatoxin in testa arachis.

关 键 词：花生衣 黄曲霉毒素 高效液相色谱-柱后光化学衍生法 

分 类 号：R927.1[医药卫生—药学]