人肝癌组织及小鼠肝损伤组织中KCTD10的表达分析研究  

Expression of KCTD10 in Human Hepatocellular Carcinoma and Injured Mouse Liver Tissues

作　　者：肖轶卉 樊安方 王道远 张浩睿 任凯群[1] XIAO Yi-hui;FAN An-fang;WANG Dao-yuan;ZHANG Hao-rui;REN Kai-qun(The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province,Medical College,Hunan Normal University,Changsha 410013,Hunan,China;Panzhihua College,Panzhihua 617000,Sichuan,China)

机构地区：[1]湖南师范大学医学院模式动物与干细胞湖南省重点实验室,中国湖南长沙410013 [2]攀枝花学院,中国四川攀枝花617000

出　　处：《生命科学研究》2022年第1期26-33,共8页Life Science Research

基　　金：国家自然科学基金资助项目(3150913);湖南省自然科学基金项目(2015JJ6072,2020JJ4441)。

摘　　要：为了探讨KCTD10在肝细胞癌(hepatocellular carcinoma, HCC)中的临床意义,本研究使用UALCAN数据库及免疫组织化学(immunohistochemistry, IHC)等方法分析KCTD10在正常组织和肝癌细胞系以及临床肝癌组织中的表达,并通过3,5-二乙酯基-1,4-二氢三甲基吡啶(3,5-diethoxycarbonyl-1,4-dihydrocollidine,DDC)饮食诱导法建立小鼠肝损伤模型,对比研究KCTD10的表达变化。结果发现,相对于癌旁组织, KCTD10在HCC组织中的表达较高,与临床分期呈正相关,且在HCC组织细胞质中表达增强,在细胞核中的表达相对降低。在肝癌细胞系Hep3B、Huh7和MHCC97H中, KCTD10的m RNA和蛋白质水平较高,且在高转移肝癌细胞株MHCC97H细胞质中最高。DDC处理后,肝细胞损伤导致KCTD10在肝细胞核中表达降低。上述结果表明, KCTD10的表达与HCC的发生发展呈正相关, KCTD10在肝癌细胞中的表达水平和部位的改变可为肝癌早期临床诊断提供理论依据。To explore the clinical significance of KCTD10 in hepatocellular carcinoma(HCC), the protein and m RNA levels of KCTD10 in normal and HCC tissues, as well as different HCC cell lines, were analyzed by UALCAN database and with methods of immunohistochemistry(IHC), quantitative real-time PCR and Western-blot. 3,5-Diethoxycarbonyl-1,4-dihydrocollidine(DDC) diet was used to induce a mouse model of liver injury, and the expression of KCTD10 in the liver was detected by IHC. The results showed that KCTD10 expression was significantly up-regulated in HCC tissues compared with paracancerous tissues. Moreover, its expression was positively correlated with the malignant grades of HCC, and was found to increase in the cytoplasm but relatively decrease in the nucleus of HCC tissues. In Hep3 B, Huh7, and MHCC97 H cell lines,both mRNA and protein levels of KCTD10 were increased, and the highest expression level of KCTD10 was detected in the cytoplasm of MHCC97 H cells. The protein level of KCTD10 was also decreased in the nucleus of injured mouse liver tissues. Together, KCTD10 expression has a positive correlation with the occurrence and development of HCC. The expression level and location change of KCTD10 in HCC cells may provide support for early clinical diagnosis of liver cancer.

关 键 词：KCTD10 肝细胞癌(HCC) 3 5-二乙酯基-1 4-二氢三甲基吡啶(DDC) 原发性肝癌等级 免疫组化(IHC) 

分 类 号：Q136[生物学—普通生物学] R735.7[医药卫生—肿瘤]