金柚幼果黄酮对AAPH诱导红细胞氧化损伤的保护作用  

Protective Effect of Flavonoids from Young Fruit of Golden Pomelo on Oxidative Damage of Erythrocyte Induced by AAPH

作　　者：纳雨农 陈乃驿 张涣悠 王琴[1,2,3] 肖更生 刘袆帆 NA Yunong;CHEN Naiyi;ZHANG Huanyou;WANG Qin;XIAO Gengsheng;LIU Huifan(College of Light Industry and Food Technology,Zhongkai University of Agricultural and Engineering,Guangzhou 510550,China;Zhongkai University&Meizhou lndustrial Zone Research Institute,Meizhou 514799,China;Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,Guangzhou 510550,China)

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510550 [2]仲恺广梅研究院,广东梅州514799 [3]广东省岭南特色食品科学与技术重点实验室,广东广州510550

出　　处：《现代食品科技》2022年第2期36-45,103,共11页Modern Food Science and Technology

基　　金：国家自然科学基金项目(32001622);广东省岭南特色食品科学与技术重点实验室(2021B1212040013)。

摘　　要：该研究通过微波超声辅助提取法提取金柚幼果粗黄酮,经AB-8大孔树脂及制备液相色谱仪纯化得到金柚幼果黄酮(PF)。使用液相色谱质谱联用仪(LC-MS)鉴定了PF的组成成分,检测结果显示PF含有35种成分,其中美鼠李苷、水苏碱、2-O-咖啡基熊果苷、甜菜碱的含量分别为15.57%、1.6%、1.15%、0.13%。在AAPH诱导的红细胞溶血模型中,经过高浓度PF处理后的实验组溶血率为31.63%,与AAPH组52.79%对比溶血率显著下降;酶促抗氧化体系中,经高浓度的PF处理后的过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的酶活性分别为241.04 U/L、93.29 U/L、72.93 U/L均低于AAPH组;非酶促抗氧化体系中,经高浓度的PF处理后的谷胱甘肽(GSH)、丙二醛(MDA)的含量分别为1233.94μmol/L、27.39 nmol/L,乳酸脱氢酶(LDH)酶活为314.75 U/L且均表现出剂量反应。由结果可知,PF主要是通过非酶促体系清除自由基,从而抑制AAPH诱导产生的氧化应激反应,此研究为金柚幼果天然产物的开发提供一定的研究基础。In this study,microwave-assisted ultrasonic were used to extracted flavonoids from golden pomelo,after purified by AB-8 macroporous resin and preparative liquid chromatography,the components of PF were identified by liquid chromatography-mass spectrometry(LC-MS).The results showed that PF contained 35 components,among which the contents of cascaroside A,stachydrine,2-O-caffeinated arbutin and betaine were 15.57%,1.6%,1.15%and 0.13%,respectively.In the model of erythrocyte hemolysis induced by AAPH,the hemolysis rate of the experimental group was 31.63%after high concentration treatment of PF,which was significantly lower than that of the AAPH group(52.79%).In the enzymatic antioxidant system,the enzyme activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT),after high concentration treatment,were 241.04 U/L,93.29 U/L and 72.93 U/L,respectively,which were lower than those in AAPH group.In the non-enzymatic antioxidant system,the contents of glutathione(GSH)and malondialdehyde(MDA)after high concentration of PF treatment were 1233.94μmol/L,27.39 nmol/L,respectively,and the activity of lactate dehydrogenase(LDH)was 314.75 U/L,and all showed a does response.The result shows that PF can inhibit AAPH induced oxidative stress mostly by scavenging free radicals through non-enzymatic system,which provides a certain research basis for the development of natural products of young pomelo.

关 键 词：金柚黄酮 红细胞溶血模型 抗氧化活性 功能性物质分析 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]