酶联免疫法检测猪肉中的司帕沙星  

Detection of Sparfloxacin in Pork by Enzyme Linked Immunosorbent Assay

作　　者：邢广旭 赵东 孙雪峰 温留丁 张颖硕 张改平 XING Guangxu;ZHAO Dong;SUN Xuefeng;WEN Liuding;ZHANG Yingshuo;ZHANG Gaiping(Henan Academy of Agricultural Sciences,Key Laboratory of Animal Immunization,Zhengzhou 450003,China)

机构地区：[1]河南省农业科学院,动物免疫学重点实验室,河南郑州450003

出　　处：《现代食品科技》2022年第2期266-271,共6页Modern Food Science and Technology

基　　金：国家研发计划重点专项(2019YFC1605700)。

摘　　要：为了建立一种检测猪肉中司帕沙星(SPFX)残留的方法,并且在检测时具有特异性高,背景低和无需样品预处理的特点。该研究制备了间接竞争性酶联免疫吸附剂和特异性抗司帕沙星单克隆抗体。结果表明,该抗体与氟甲喹(11.99%)、氟罗沙星(11.14%)、恩诺沙星(4.60%)、环丙沙星(0.31%)几乎没有交叉反应。该方法测定了添加SPFX的猪肉中的药物残留,检测内变异系数小于5.68%,检测间变异系数小于5.51%。检测间和检测内的平均回收率分别为102%~106%和101%~104%。该抗体对SPFX的IC_(50)为31μg/L,没有抗体具有四种结构相关化合物和其他化合物。高效液相色谱(HPLC)和酶联免疫吸附试验(ELISA)的结果进一步证实了icELISA快速检测猪肉中SPFX的可靠性和准确性。该研究表明icELISA法检测猪肉中SPFX残留分析是一种可靠、简便、低成本的方法。To establish a method for detecting sparfloxacin(SPFX)residues in pork,which has the characteristics of high specificity,low background,and no need for sample pretreatment,an indirect competitive enzyme-linked immunosorbent and a specific anti-spafloxacin monoclonal antibody were prepared in this paper.The results showed that the antibody had almost no cross-reactivity with flumequine(11.99%),fleroxacin(11.14%),enrofloxacin(4.60%),and ciprofloxacin(0.31%).This method measured drug residues in pork with SPFX,and the intra-detection coefficient of variation was less than 5.68%,and the inter-detection coefficient of variation was less than 5.51%.The average recovery rates between and within the test were 102%~106%and 101%~104%,respectively.The IC_(50) of this antibody against SPFX is 31μg/L,and almost no antibody has four structurally related compounds and other compounds.The results of high-performance liquid chromatography(HPLC)and enzyme-linked immunosorbent assay(ELISA)further confirmed the reliability and accuracy of the rapid detection of SPFX in pork by icELISA.This study shows that the icELISA method to detect SPFX residues in pork is a reliable,simple,and low-cost method.

关 键 词：司帕沙星 单克隆抗体 酶联免疫吸附试验 猪肉 

分 类 号：S859.84[农业科学—临床兽医学]