沙门氏菌跨物种感染基因的挖掘及鉴定  

Discovery and identification of salmonella cross-species infection genes

作　　者：管延鹏 崔灵芝 庄倩倩 刘新利[1] GUAN Yan-peng;CUI Ling-zhi;ZHUANG Qian-qian;LIU Xin-li(School of Bioengineering,Shandong Provincial Key Laboratory of Microbial Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学院,山东省微生物工程重点实验室,济南250353

出　　处：《齐鲁工业大学学报》2022年第1期7-11,共5页Journal of Qilu University of Technology

基　　金：国家自然科学基金(21808114);山东省重点研发计划(2019GSF107044)。

摘　　要：沙门氏菌,肠杆菌科是导致人兽共患病的重要病原体。微生物的跨物种感染是全世界研究的热点。本实验选用从鸡蛋和病人中分离的30种肠炎沙门氏菌菌株进行全基因组测序,使用Ridom SeqspHere+进行cgMLST分析,挖掘可能的人兽共患性致病基因,分别为invA、pduD、kduI、yigM、mgtA,并以这些靶基因为模板,设计PCR引物,建立五重PCR鉴定沙门氏菌致病基因的方法。以包含上述5个基因的G1菌株作为阳性检测菌株,并对建立的五重PCR进行检测,结果显示均扩增出目的条带且无杂带,说明建立的五重PCR检测方法较为成功。该检测方法能够同时鉴定沙门氏菌产生的多种毒力基因,具有特异性强、灵敏度高的特点,为沙门氏菌菌株的检测和鉴定提供了一种新的方法。Salmonella,Enterobacteriaceae,is an important pathogen causing zoonotic diseases.Cross-species infection of microorganisms is a hot research topic all over the world.In this experiment,30 strains of Salmonella enteritidis isolated from eggs and patients were used for whole-genome sequencing,and Ridom SeqspHere+was used for cgMLST analysis to discover possible zoonotic pathogenic genes,namely invA,pduD,kduI,yigM,mgtA,and these target genes were used as templates to design PCR primers and a five-fold PCR method was established to identify Salmonella pathogenic genes.The G1 strain containing the above five genes was used as the positive detection strain,and the established five-fold PCR was tested.The results showed that the target bands were amplified and there were no confounding bands,indicating that the established five-fold PCR detection method was relatively successful.The detection method can simultaneously identify multiple virulence genes produced by Salmonella,with strong specificity and high sensitivity,and provide a new method for the detection and identification of Salmonella strains.

关 键 词：沙门氏菌 人兽共患性致病基因 多重PCR鉴定 

分 类 号：R978.16[医药卫生—药品]