低强度脉冲超声靶向纳米粒控释SDF-1及BMP-2调节hPDLCs迁移和成骨分化  

Low-intensity Pulsed Ultrasound-targeted Nanoparticles Controlled-release SDF-1 and BMP-2 to Regulate Migration and Osteogenic Differentiation of hPDLCs

作　　者：晏殊瑾 杨珂[2] 王河 张亮[1] 甘甜 姚欢 王冬[1] Yan Shujin;Yang Ke;Wang He;Zhang Liang;Gan Tian;Yao Huan;Wang Dong(Department of Ultrasound,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;Pediatric Research Institute,Children's Hospital of Chongqing Medical University,Chongqing 400010,China;Department of Orthodontics,Stomatological Hospital of Chongqing Medical University,Chongqing 400010,China;Chongqing Key Laboratory of Ultrasound Molecular Imaging,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第一医院超声科,重庆市400010 [2]重庆医科大学附属儿童医院儿科研究所,重庆市400010 [3]重庆医科大学附属口腔医院正畸科,重庆市400010 [4]超声分子影像重庆市重点实验室,重庆市400010

出　　处：《中国超声医学杂志》2022年第2期222-226,共5页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金(No.81771845)。

摘　　要：目的该文主要研究低强度脉冲超声(LIPUS)靶向PEG-PLGA纳米粒控释SDF-1α、BMP-2对人牙周膜干细胞(hPDLCs)迁移和成骨分化的调节作用。方法以双乳化法制备SDF-1α/BMP-2/PEG-PLGA,检测其基本表征,BCA蛋白定量法检测蛋白释放情况。实验分为对照组、SDF-1α+BMP-2组、SDF-1α/BMP-2/PEG-PLGA组和LIPUS+SDF-1α/BMP-2/PEG-PLGA组。Transwell细胞迁移实验检测各组迁移能力,碱性磷酸酶(ALP)、茜素红染色实验和实时定量PCR评估各组细胞成骨分化能力。结果LIPUS辐照成功调节了纳米粒内蛋白的释放速率。与其他组相比,LIPUS+SDF-1α/BMP-2/PEG-PLGA组迁移能力和成骨分化能力显著增强。结论LIPUS靶向作用PEG-PLGA纳米粒成功调节了SDF-1α、BMP-2的释放,该体系能有效促进人牙周膜干细胞迁移和成骨分化。Objective To investigate regulatory effect of low-intensity pulsed ultrasound(LIPUS)-targeted PEG-PLGA nanoparticles controlled-releasing of SDF-1、BMP-2 on migration and osteogenic differentiation of human periodontal ligament stem cells(hPDLCs).Methods Nanoparticles were prepared by double emulsion solvent evaporation method(W/O/W).Their basic physicochemical properties and protein release rate were detected.The experimental groups were:control group,SDF-1α+BMP-2 group,SDF-1α/BMP-2/PEG-PLGA group,LIPUS+SDF-1α/BMP-2/PEG-PLGA group.The migration and osteogenic differentiation efficacy were evaluated in all groups.Results LIPUS successfully regulated the protein release rate of nanoparticles.Migration and osteogenic differentiation in LIPUS+SDF-1α/BMP-2/PEG-PLGA group were significantly enhanced compared to other groups.Conclusions LIPUS successfully regulated the protein release rate of PEG-PLGA nanoparticles.This new drug delivery system is more effective in promoting migration and osteogenic differentiation.

关 键 词：低强度脉冲超声 蛋白缓释 超声控释 人牙周膜干细胞 成骨分化 

分 类 号：R781.42[医药卫生—口腔医学] R445.1[医药卫生—临床医学]