毒蕈碱受体通过Yes相关蛋白信号对口腔鳞状细胞癌生物学行为的实验研究  

Experimental study of muscarinic receptors on the biological behavior of oral squamous cell carcinoma through Yes related protein signal

作　　者：江涵 神应强 陈谦明 Jiang Han;Shen Yingqiang;Chen Qianming(State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&National Key Laboratory of Oral Mucosal Diseases&Innovation Unit of Oral Mucosal Carcinogenesis and Prevention,Chinese Academy of Medical Sciences&Dept.of Oral Medicine,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,口腔黏膜病国家临床重点专科,中国医学科学院口腔黏膜癌变与防治创新单元,四川大学华西口腔医院口腔黏膜病科,成都610041

出　　处：《国际口腔医学杂志》2022年第2期138-143,共6页International Journal of Stomatology

基　　金：四川大学华西口腔医学院(华西口腔医院)科研经费资助项目(RCDWJS2020-20)。

摘　　要：目的研究毒蕈碱(m)受体在口腔鳞状细胞癌(OSCC)中发挥的作用及机制。方法分析癌症基因图谱(TCGA)数据库,并利用实时定量聚合酶链式反应(RT-qPCR)荧光定量查询和检测m受体基因在头颈部肿瘤和OSCC细胞中的表达水平,再采用m受体激动剂卡巴胆碱进一步研究其对OSCC细胞增殖和侵袭的影响,最终利用免疫荧光探究激活m受体对OSCC细胞内Yes相关蛋白(YAP)核转位的影响。结果m受体基因在头颈部肿瘤中有表达差异:在5种OSCC细胞系中HN4和HN12的m3受体基因高表达(P<0.05)。激活m受体具有促进OSCC细胞增殖和侵袭的潜能,促进YAP的核转位可能是m受体的作用机制。结论m受体在OSCC发生发展中发挥重要作用,靶向m受体可能是未来治疗OSCC的新策略。Objective This study aims to study the role and mechanism of muscarinic(m)receptors in oral squamous cell carcinoma(OSCC).Methods The study involved a query on The Cancer Gene Atlas(TCGA)database of head and neck cancer and used real-time quantitative polymerase chain reaction(RT-qPCR)to detect the expression level of m receptor genes in OSCC cells.The m receptor agonist Carbachol was used to further study the proliferation of OSCC cells.Immunofluorescence was used to explore the influence of activated m receptor on the localization of Yes related protein(YAP)in OSCC cells.Results The expression of m receptor genes was different in HNSC.Specifically,chrm3 was highly expressed in HN4 and HN12(P<0.05).The activation of m receptor can promote OSCC cell proliferation and invasion.Promoting the nuclear translocation of YAP may be the mechanism of m receptor.Conclusion The m receptors are essential for the occurrence and development of OSCC,and targeting m receptors may be a new strategy for the treatment of OSCC in the future.

关 键 词：毒蕈碱受体 口腔鳞状细胞癌 卡巴胆碱 

分 类 号：R739.8[医药卫生—肿瘤]