高通量测序分析牙周基础治疗对65岁以上糖尿病合并慢性牙周炎患者龈下菌群的影响  

Effect of periodontal basic treatment on subgingival microflora in patients over 65 years old with diabetes mellitus and chronic periodontitis by high throughput sequencing analysis

作　　者：牛家慧[1] 李创[1] 李蓉 胡永权[1] 张江畔 苑学微 樊静珊 张亚宏[1] NIU Jiahui;LI Chuang;LI Rong;HU Yongquan;ZHANG Jiangpan;YUAN Xuewei;FAN Jingshan;ZHANG Yahong(Department of Stomatology,Shijiazhuang Second Hospital,Shijiazhuang 050051,China)

机构地区：[1]石家庄市第二医院口腔科,石家庄050051

出　　处：《临床与病理杂志》2022年第2期346-351,共6页Journal of Clinical and Pathological Research

基　　金：河北省重点研发计划(192777101D)。

摘　　要：目的:采用高通量测序技术分析牙周基础治疗对65岁以上糖尿病合并慢性牙周炎(diabetes mellitus with chronic periodontitis,DMCP)患者龈下菌群的影响。方法:选取100例65岁以上DMCP患者作为研究对象,采用随机数据表法将患者分为研究组与对照组,每组50例。对照组给予强化控制血糖、基础疾病对症治疗、口腔卫生知识宣教等常规治疗,研究组在此基础上给予牙周基础治疗,治疗及复诊在4周内完成。比较2组患者的基线资料,采用高通量测序技术比较2组患者治疗前、治疗后3个月时牙周龈下菌群中致病菌的相对含量。结果:研究组与对照组分别有2例和5例患者失访,最终分别纳入48例和45例患者。2组患者各项基线资料的差异均无统计学意义(均P>0.05)。2组患者治疗前牙周龈下菌群中各类病原菌相对含量及牙周龈沟液中各种氧化还原物质水平的差异无统计学意义(P>0.05)。在治疗后3个月时,2组患者牙周龈下菌群中各类病原菌相对含量均较治疗前下降,差异均有统计学意义(P>0.05),研究组牙周龈下菌群中各类病原菌相对含量均低于对照组,差异均有统计学意义(P>0.05)。结论:牙周基础治疗能够显著减少DMCP患者牙周龈下菌群中致病菌的含量,纠正口腔菌群失调。Objective:To analyze the effects of basic periodontal treatment on the subgingival microflora of patients over 65 years old with diabetes mellitus and chronic periodontitis(DMCP)by high throughput sequencing technology and to analyze the mechanism of oxidative stress.Methods:A total of 100 DMCP patients over 65 years old were selected as the research subjects and randomly divided into a study group and a control group,with 50 cases in each group.The patients in the control group were treated with the routine treatments,such as intensive blood glucose control,symptomatic treatment of basic diseases and oral health knowledge education while the patients in the study group were treated with periodontal basic treatment on this basis.The treatments and follow-up visit were completed within 4 weeks.The baseline data between the 2 groups were compared;the relative contents of periodontal subgingival bacteria before and at 3 months after the treatments between the 2 groups were compared by high-throughput sequencing technology.Results:Two patients in the study group and 5 patients in the control group were lost to follow-up,and finally 48 patients and 45 patients were included.There was no significant difference in baseline data between the 2 groups(P>0.05).There was no significant difference between the 2 groups in the relative contents of various pathogenic bacteria in the periodontal subgingival microflora and the levels of various oxidizing and reducing agents in the gingival crevicular fluid before treatment(P>0.05).Three months after the treatment,the relative contents of all kinds of pathogenic bacteria in periodontal subgingival flora of 2 groups were decreased compared with that before treatment,and the differences were statistically significant(P>0.05).The relative contents of all kinds of pathogenic bacteria in periodontal subgingival flora of the study group were lower than that of the control group,and the differences were statistically significant(P>0.05).Conclusion:The application of periodontal basic trea

关 键 词：高通量测序 牙周基础治疗 糖尿病合并牙周炎 龈下菌群 氧化应激 

分 类 号：R781.42[医药卫生—口腔医学] R587.2[医药卫生—临床医学]