响应面法优化辣木叶蛋白提取工艺及凝集活性研究  

Optimization of Extraction Process and Agglutination Activity of Moringa oleifera Leaf Protein by Response Surface Methodology

作　　者：杨豪 刘晓雪 苏海冉 李凌飞 宋爽 YANG Hao;LIU Xiaoxue;SU Hairan;LI Lingfei;SONG Shuang(College of Food Science and Technology,Yunnan Agricultural University,National Moringa Processing Technology R&D Center,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,国家辣木加工技术研发专业中心,云南昆明650201

出　　处：《食品工业科技》2022年第6期150-157,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(31960508)。

摘　　要：辣木叶中含有丰富的蛋白质,但溶解性较差,本研究采用盐析法提取新鲜辣木叶中的蛋白并对其活性进行研究。以新鲜辣木叶为原料,分析提取时间、硫酸铵饱和度、料液比3个因素对蛋白得率的影响,在单因素实验的基础上,通过响应面优化盐析法提取辣木叶蛋白的最佳工艺条件。结果表明:对蛋白得率的影响依次为提取时间>硫酸铵饱和度>料液比,确定的最佳工艺条件为提取时间7 h、硫酸铵饱和度71%、料液比1:9 g/mL,在此条件下辣木叶蛋白得率为13.49 mg/g,与预测结果13.76 mg/g基本相符。辣木叶蛋白的凝集效价为2^(8),D(+)无水葡萄糖是辣木凝集素专一性抑制糖,可以较好地抑制兔红细胞和辣木叶蛋白凝集反应。Moringa leaves are rich in protein,but less soluble.In this study,the protein from fresh Moringa leaves was extracted by salting out method and its activity was studied.Using fresh Moringa leaves as raw material,the effects of extraction time,ammonium sulfate saturation,and solid-liquid ratio on the protein yield were analyzed.On the basis of single-factor experiments,the response surface methodology was used to optimize the extraction of Moringa oleifera protein by salting out method optimum process conditions.The results showed that the effects on protein yield were in order of extraction time>ammonium sulfate saturation>solid-liquid ratio,and the optimal process conditions were determined as extraction time 7 h,ammonium sulfate saturation 71%,and solid-liquid ratio 1:9 g/mL,under this condition,the yield of Moringa leaf protein was 13.49 mg/g,which was basically consistent with the predicted result of 13.76 mg/g.The agglutination titer of Moringa oleifera protein was 2^(8),and D(+)anhydrous glucose was a specific inhibitory sugar of Moringa lectin,which could better inhibit the agglutination reaction of rabbit erythrocytes and Moringa oleifera protein.

关 键 词：辣木叶蛋白 提取 工艺优化 凝集活性 糖抑制作用 

分 类 号：S794.9[农业科学—林木遗传育种]