糯玉米耐贮藏相关性状的主效QTL定位  

Major QTL mapping for storability related traits in waxy corn

作　　者：蒋锋 刘伟杰 陈青春 张姿丽 孙伟 刘鹏飞 JIANG Feng;LIU Weijie;CHEN Qingchun;ZHANG Zili;SUN Wei;LIU Pengfei(College of Agronomy and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Agricultural and Rural Office of Shiling Town,Huadu District,Guangzhou 510326,China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广州510225 [2]广州市花都区狮岭镇农业农村办公室,广州510326

出　　处：《中国农业大学学报》2022年第2期15-21,共7页Journal of China Agricultural University

基　　金：广东省鲜食玉米遗传育种工程技术研究中心(粤科产学研[2016]176号);广东省教育厅育苗工程项目(2012LYM_0078);广州市特色作物种质资源研究与利用重点实验室项目(202002010010)。

摘　　要：为挖掘、利用玉米耐贮藏基因资源,对糯玉米组合N7×N32的216个F_(2)单株种子进行2年的常温贮藏,并以贮藏后种子的单位质量电导率和发芽率为耐贮藏性指标,建立极端耐与不耐贮藏BSA混池。首先筛选在2个BSA混池间有多态性的引物,后在多态引物所在的染色体上增加引物,以F_(2)为作图群体,构建分子标记连锁图谱;同时结合测得的F_(2)耐贮藏性状数据,利用复合区间作图法检测耐贮藏相关QTL。结果表明:1)从600对SSR引物中筛选出2对在BSA混池间有多态性的引物,分别位于第4和6染色体上;2)在第4和6染色体上增加200对SSR引物,构建有39个标记位点总长为234.9cM的SSR标记连锁图谱;3)共检测到7个耐贮藏相关QTL。其中,在第4染色体区间bnlg1265-bnlg1755内同时定位到2个指标的QTL(qCE-4-1和qGP-4-1),LOD值分别为19.3和18.1,贡献率分别为30.5%和25.9%;在第6染色体umc1014-bnlg1154内也同时定位到2个指标的QTL(qCE-6-1和qGP-6-1),LOD值分别为11.2和8.2,贡献率分别为15.0%和10.3%。本研究可为耐贮藏QTL的精细定位、分子标记辅助选择耐贮藏新品种提供理论依据。To explore and utilize storability gene resources,216 F_(2) ear seeds of waxy maize combination N7×N32were stored at room temperature for 2years.The electroconductibility per weight and germination percentage of F_(2) seeds after storage were used as storability indexes,and the DNA pool of extreme storability was formed.Primers with polymorphism between the two BSA pools were screened,molecular marker linkage map was constructed by adding primers on chromosomes of polymorphic primers using F_(2)as the mapping population.Furthermore,combined with the seed storability data of 216 F_(2) individuals,QTL related to storability were detected by composite interval mapping.The results showed that:1)Two SSR primers with polymorphism between DNA pools were found from 600primers,which were located on chromosomes 4and 6,respectively;2)A SSR linkage map containing 39markers with a total length of 234.9cM was constructed by adding 200primers on chromosomes 4and 6;3)Seven QTL were detected on chromosomes 4and 6.Among them,two QTL(qCE-4-1 and qGP-4-1)of two indexes were simultaneously located in the region of bnlg1265-bnlg1755on chromosome 4,with LOD values of 19.3and 18.1,accounting for 30.5% and 25.9% of phenotypic variation,respectively.Moreover,two QTL (qCE-6-1and qGP-6-1)were simultaneously screened in umc1014-bnlg1154of chromosome 6,with LOD values of 11.2and 8.2,accounting for 15.0% and 10.3% of phenotypic variation,respectively.This study provides a theoretical basis for the fine mapping of QTL and molecular marker assisted selection of new storability varieties.

关 键 词：糯玉米 耐贮藏性 QTL 

分 类 号：S513[农业科学—作物学]