星状神经节阻滞术前处理减轻脑缺血再灌注大鼠的脑梗死  

Pretreatment with stellate ganglion block alleviates cerebral infarction in the rats with cerebral ischemia-reperfusion

作　　者：常新会[1] 司海超[2] 徐宏超[2] Chang Xinhui;Si Haichao;Xu Hongchao(Department of Critical Care Medicine,Nanyang Central Hospital,Nanyang 473000;Department of Anesthesiology,NanyangCentral Hospital,Nanyang 473000)

机构地区：[1]南阳市中心医院重症医学科,南阳473000 [2]南阳市中心医院麻醉科,南阳473000

出　　处：《中国组织化学与细胞化学杂志》2021年第5期442-448,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：2019年南阳市科技计划项目(KJGG177)。

摘　　要：目的探讨星状神经节阻滞术(stellate ganglion block,SGB)前处理对脑缺血/再灌注(ischemia reperfusion,I/R)模型大鼠的治疗作用以及相关机制。方法将SD大鼠随机分为3组:假手术组(Sham)、脑I/R组和I/R+SGB组。采用大脑中动脉阻塞术模拟大鼠脑I/R损伤,并在I/R术前进行SGB处理。I/R术后7d,用激光多普勒血流仪测量局部脑血流量;用TTC染色测量脑梗死体积;用HE染色观察缺血半影区组织形态学;用TUNEL染色法检测缺血半影区中神经元凋亡水平;用透射电镜观察缺血半影区中神经元细胞自噬;用Western blot检测缺血半影区中自噬和凋亡相关蛋白的表达水平。结果SGB前处理可减轻I/R引起的病理性脑损伤,恢复局部脑血流,并能降低I/R后缺血半影区神经元的凋亡和自噬水平。结论SGB前处理可改善大鼠局灶性脑I/R损伤,并减轻缺血半影区神经元凋亡和自噬反应,其机制可能与抑制Cleaved Caspase-3、beclin 1和LC3表达有关。Objective To investigate the therapeutic effect of stellate ganglion block(SGB)pretreatment on cerebral ischemia/reperfusion(I/R)rat model and its related mechanisms.Methods SD rats were randomly divided into three groups:sham operation group(Sham),cerebral ischemia/reperfusion(I/R)group and I/R+SGB group.Middle cerebral artery occlusion was used to simulate cerebral I/R injury in rats,and SGB treatment was performed before I/R.At 7 days after I/R operation,regional cerebral blood flow was measured by laser Doppler flowmeter;cerebral infarct size was measured by TTC staining;the histomorphology of ischemic penumbra was observed by HE staining;the neuronal apoptosis level in ischemic penumbra was detected by TUNEL staining;autophagy of neurons in ischemic penumbra was observed by transmission electron microscopy;the expression levels of autophagy-and apoptosis-related proteins in ischemic penumbra were detected by Western blot.Results SGB pretreatment reduced the pathological cerebral injury caused by I/R,restored regional cerebral blood flow,and reduced the levels of neuronal apoptosis and autophagy in the ischemic penumbra after I/R of the rat model.Conclusion SGB pretreatment can ameliorate the focal brain I/R injury,and reduce the neuronal apoptosis and autophagy in the ischemic penumbra,the mechanism of which may be related to the inhibition of the expression of Cleaved Caspase-3,beclin 1 and LC3.

关 键 词：星状神经节阻滞术 脑缺血再灌注损伤 自噬 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]