虎杖多糖通过NF-κB/P65信号通路抑制卵巢癌细胞增殖  

Polygonum Cuspidatum Polysaccharide Inhibits the Proliferation of Ovarian Cancer Cells through NF-κB/P65 Signaling Pathway

作　　者：曾博洁 许剑利[1] 董珊[1] ZENG Bojie;XU Jianli;DONG Shan(Department of Gynecology,Zigong First People′s Hospital,Zigong 643000,China)

机构地区：[1]自贡市第一人民医院妇科,自贡643000

出　　处：《宁夏医科大学学报》2022年第2期109-113,121,共6页Journal of Ningxia Medical University

摘　　要：目的探究虎杖多糖抑制卵巢癌细胞增殖的分子机制。方法采用MTT法和菌落形成实验分析细胞增殖。采用qRT-PCR法检测SKOV3细胞中P65mRNA表达水平。采用免疫印迹检测P65蛋白表达水平。采用流式细胞仪检测细胞周期和细胞凋亡率。采用TUNEL检测细胞凋亡。结果虎杖多糖抑制卵巢癌细胞增殖,并且促进其凋亡。虎杖多糖诱导SKOV3细胞中P65的mRNA及蛋白表达水平降低。虎杖多糖诱导SKOV3细胞中S期阻滞。结论虎杖多糖通过NF-κB/P65信号通路抑制卵巢癌细胞增殖,并促进其凋亡。Objective To explore the molecular mechanism of polygonum cuspidatum polysaccharide inhibiting the proliferation of ovarian cancer cells.Methods MTT method and colony formation test were used to detect cell proliferation.The qRT-PCR method was used to detect the expression level of P65 mRNA in SKOV3 cells.Western blot was used to detect the expression level of P65 protein.Flow cytometry was used to detect cell cycle and cell apoptosis rate.TUNEL was used to detect cell apoptosis.Results Polygonum cuspidatum polysaccharide inhibited the proliferation of ovarian cancer cells and promoted their apoptosis.Polygonum cuspidatum polysaccharide induced the increase of P65 mRNA and protein expression in SKOV3 cells.Polygonum cuspidatum polysaccharide induces S-phase arrest in SKOV3 cells.Conclusion Polygonum cuspidatum polysaccharide can inhibit the proliferation of ovarian cancer cells and promotes their apoptosis through the NF-κB/P65 signaling pathway.

关 键 词：虎杖多糖 迁移和侵袭 卵巢癌 P65 

分 类 号：R737.31[医药卫生—肿瘤]