锌指和同源框2在肝母细胞瘤中的表达及临床意义  

Expression and clinical significance of zinc-finger and homeoboxes 2 in hepatoblastoma

作　　者：杜升园 韦宇音 吕自力[1] Du Shengyuan;Wei Yuyin;Lyu Zili(Department of Pathology,First Clinical Medical College,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一临床医学院病理科,南宁530021

出　　处：《广西医科大学学报》2022年第2期323-329,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.8186100164)。

摘　　要：目的:探讨锌指和同源框2(ZHX2)在肝母细胞瘤(HB)中的表达情况及临床意义。方法:收集基因表达综合数据库(GEO)、arrayExpress等外文数据库中ZHX2在HB中的表达值进行综合分析,采用实时荧光定量PCR技术(RT-qPCR)验证HB细胞系(HuH-6)与正常肝细胞系(LO2)中ZHX2的表达,免疫组化技术验证50例HB组织和50例非瘤肝组织中ZHX2的表达情况。结果:ZHX2在瘤组织中的表达高于非瘤组织(P<0.05),ZHX2蛋白的表达与HB患者性别、年龄、组织学类型和AFP差异无统计学意义(P>0.05),与肿瘤大小及PRETEXT临床分期差异有统计学意义(P<0.05)。结论:ZHX2在HB组织中表达较高,可能是HB的致癌因子,有望为HB诊疗提供新的思路。Objective:To investigate the expression of zinc-fingers and homeoboxes 2(ZHX2)in hepatoblastoma(HB)and its clinical value.Methods:The expression values of ZHX2 in HB were collected from the Gene Expression Omnibus(GEO)and arrayExpress databases for comprehensive analysis.The expression of ZHX2 in the HB cell line(HuH-6)and normal liver cell line(LO2)was verified by real-time quantitative PCR(RT-qPCR).The expression of ZHX2 in 50 HB tissues and 50 non-tumor liver tissues was verified by immunohistochemistry.Results:The expression of ZHX2 was visibly higher in tumor tissues than that in non-tumor tissues(P<0.05).The difference in the protein expression of ZHX2 was not statistically significant with gender,age,histological type and serum alpha-fetoprotein(AFP)of HB patients(P>0.05).However,it was statistically significant with tumor size and PRETEXT staging(P<0.05).Conclusion:ZHX2 is highly expressed in HB tissues and may be a carcinogenic factor of HB,which is expected to provide new insights for diagnosis and treatment of HB tumors.

关 键 词：肝母细胞瘤 ZHX2 基因芯片 RT-QPCR 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]