双侧颈总动脉结扎对大鼠大脑皮质小胶质细胞活化的影响  

The effects of bilateral common carotid artery occlusion on microglia cell activation in the cerebral cortex of rat

作　　者：邓汝东[1] 周贤熙 周丽亭 姚玉宏 张赛霞[1] 伍趣京[1] Deng Rudong;Zhou Xianxi;Zhou Liting;Yao Yuhong;Zhang Saixia;Wu Qujing(Center for Experimental Teaching,School of Basic Medical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006;Zhanjiang Traditional Chinese Medicine Hospital,Zhanjiang 524000,China)

机构地区：[1]广州中医药大学基础医学院实验教学中心,广州510006 [2]湛江市第一中医医院,湛江524000

出　　处：《神经解剖学杂志》2021年第6期677-681,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81673770)。

摘　　要：目的:研究血管性痴呆对大鼠大脑皮质小胶质细胞活化的影响.方法:36只成年雄性SD大鼠分为假手术组(sham)和双侧颈总动脉结扎组(BCCAO),利用双侧颈总动脉永久结扎的方法制备血管性痴呆大鼠模型,通过免疫组织化学染色方法检测大鼠大脑皮质蛋白离子钙接头蛋白1(Iba-1)、诱导型一氧化氮合成酶(iN-OS)和精氨酸酶-1(Arg-1)的表达,利用real time RT-PCR方法检测iNOS、Arg-1和Iba-1 mRNA表达水平变化,Western Blot方法检测iNOS、Arg-1和Iba-1蛋白的表达变化.结果:与对照组相比,免疫组织化学染色结果显示:血管性痴呆模型大鼠大脑皮质iNOS、Arg-1和Iba-1阳性细胞增多,real time RT-PCR检测结果显示:iNOS、Arg-1和Iba-1 mRNA表达水平增加,Western Blot检测结果,显示:血管性痴呆大鼠大脑皮质iNOS、Arg-1和Iba-1蛋白质表达均上调.结论:双侧颈总动脉结扎大鼠大脑皮质的小胶质细胞被激活.Objective: To study the effects of vascular dementia on microglia cell activation in vascular dementia rats. Methods: Thirty-six adult male SD rats were randomly divided into sham group(sham),bilateral common carotid artery occlusion group(BCCAO). The BCCAO rats were prepared by bilateral common carotid arteries occlusion permanently. The expression of ionized calcium binding adapter molecule-1(Iba-1), induced nitric oxide synthase(iNOS) and arginase-1(Arg-1) was detected by immunohistochemistry staining. The mRNA expression of Iba-1,iNOS and Arg-1 was detected by real time RT-PCR. The protein expression level of Iba-1,iNOS and Arg-1 was detected by Western Blot. Results: Compared with the sham group, the number Iba-1,iNOS and Arg-1 positive cells increased in BCCAO rats. The mRNA expression of Iba-1,iNOS and Arg-1 was up-regulated, meanwhile, the protein level of iNOS,Arg-1 and Iba-1 of microglia in BCCAO rats was significantly up-regulated consistently. Conclusion: Microglia activation was increased significantly in BCCAO rats.

关 键 词：血管性痴呆 小胶质细胞 神经元 大鼠 

分 类 号：R749.13[医药卫生—神经病学与精神病学]