维生素E对慢性癫痫大鼠海马氧化应激的影响  

The Effects of vitamin E on oxidative stress in hippocampus of rats with chronic epilepsy

作　　者：张鑫帆 郭翀 彭江涛 胡忠波[1] 刘睿 章朝 吴淑华[2] 李建民[1] Zhang Xinfan;Guo Chong;Peng Jiangtao;Hu Zhongbo;Liu Rui;Zhang Zhao;Wu Shuhua;Li Jianmin(Department of Neurosurgery,Binzhou Medical University Hospital,Binzhou 256603,China;Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,China)

机构地区：[1]滨州医学院附属医院神经外科,滨州256603 [2]滨州医学院附属医院病理科,滨州256603

出　　处：《神经解剖学杂志》2021年第6期695-700,共6页Chinese Journal of Neuroanatomy

基　　金：山东省医药卫生科技发展计划(2017WS553);滨州医学院科技计划项目(BY2015KJ13);滨州医学院科技计划项目(BY2020KJ13)

摘　　要：目的:研究维生素E对慢性癫痫大鼠海马组织脂氧合酶15(15-LOX)和氧化应激的影响.方法:选用雄性SD大鼠36只,随机分为3组,分别为对照组、癫痫组和维生素E处理组.采用戊四氮(PTZ)诱导的慢性癫痫大鼠模型并用维生素E干预,通过行为学观察大鼠癫痫发作程度,运用全细胞膜片钳技术分析大鼠海马神经元动作电位的变化情况,采用分光光度计法检测海马组织中丙二醛(MDA)和谷胱甘肽(GSH)含量,应用免疫荧光染色和Western Blot检测15-LOX表达情况.结果:与对照组相比,癫痫组大鼠癫痫发作明显,海马区MDA含量和15-LOX表达升高,GSH含量降低,神经元兴奋性升高(P<0.01);与癫痫组相比,维生素E处理组大鼠癫痫发作强度降低,海马区MDA和15-LOX表达明显降低,GSH含量升高,神经元兴奋性降低(P<0.01).结论:维生素E可通过抑制15-LOX降低氧化应激和神经元的兴奋性进而影响癫痫发作.Objective:To study the effects of vitamin E on 15-lipoxygenase(15-LOX)and oxidative stress in the hippocampus of rats with chronic epilepsy.Methods:Thirty-six male Sprague-Dawley rats were randomly divided into three groups:control group,epilepsy group and vitamin E treatment group.The chronic epileptic rat model induced by pentylenetetrazol(PTZ),and intervened with vitamin E.The seizure degree was observed by behavior,the changes of the action potential in hippocampal neurons were analyzed by whole-cell patch-clamp technique,the contents of malond-ialdehyde(MDA)and glutathione(GSH)in hippocampal tissue were detected by spectrophotometer,and the expression of 15-LOX was detected by immunofluorescence staining and Western Blot.Results:It’s more obvious of epileptic seizure for rats in epilepsy group compared to the control group,the content of MDA and expression of 15-LOX increased,the level of the GSH decreased and the excitability of hippocampal neurons increased significantly(P<0.01);However,when rats were injected with vitamin E compared with epilepsy group,the seizure intensity decreased,the content of MDA and expression of 15-LOX decreased obviously,the level of the GSH increased and the excitability of hippocampal neurons decreased significantly(P<0.01).Conclusion:Vitamin E can reduce oxidative stress and neuronal excitability by inhibiting 15-LOX.

关 键 词：癫痫 维生素E 脂氧合酶15 氧化应激 动作电位 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]