基于超分子“印迹模板”的鱼腥草注射剂致敏原研究  

Study on allergen of Houttuynia cordata Injection based on supramolecular “imprinted template”

作　　者：周燕子 陈锋[1,2] 王敏存 肖美凤 贺福元[1,2,3] ZHOU Yan-zi;CHEN Feng;WANG Min-cun;XIAO Mei-feng;HE Fu-yuan(College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM,Changsha 410208,China;Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208 [3]湖南中医药大学中医药超分子机理与数理特征化实验室,湖南长沙410208

出　　处：《中草药》2022年第1期154-161,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81573691);国家自然科学基金资助项目(81874507);国家自然科学基金青年项目(81803729);湖南省教育厅优秀青年项目(20B438);湖南省自然科学基金青年项目(2019JJ50430);湖南省自然科学基金面上项目(2021JJ30509)。

摘　　要：目的基于超分子"印迹模板"和非线性回归对鱼腥草注射剂致敏原进行研究。方法小鼠尾iv鱼腥草注射剂(18.1mL/kg),于不同时间点采集样本,进行气质联用(GC-MS)分析和免疫球蛋白E(Immunoglobulin E,IgE)的含量测定;从所有样本中筛选出共有成分,并将非共有成分进行归类,划分成若干个成分群;对各个成分群进行相似度计算;利用非线性回归对各个成分群与IgE的相关性进行分析,并计算信息量。结果共得到鱼腥草注射剂体内成分605个,其中共有成分10个,分别是2,4-二甲基庚烷、3,7-二甲基癸烷、2,6-二甲基癸烷、2,3-二甲基十氢萘、5-甲基十四烷、4,6-二甲基十二烷、4-甲基十二烷、十六烷、正十七烷、2,4-二叔丁基苯酚;将所有成分归类得到10个成分群;各个成分群相似度为0.934~1.000;非线性回归得到各个成分群的效应系数,进一步计算得到成分群3的信息量最大(17.67%)。结论以超分子"印迹模板"为理论指导,对鱼腥草注射剂体内成分进行了归类;利用非线性回归得到了各个成分群致敏性的排序,成分群3可能是造成致敏的关键成分群。Objective To study the sensitizer of Houttuynia cordata Injection(鱼腥草注射剂)based on supramolecular imprinting template and nonlinear regression.Methods Houttuynia cordata Injection(18.1 m L/kg)was administered to mice by tail vein injection.Samples were collected at different time points to analyze by GC-MS and determine the content of IgE.The common components were screened out from all samples,and non-common components were classified and divided into several component groups.The similarity of each component group was calculated.The correlation between each component group and IgE was analyzed by nonlinear regression,and information amount was calculated.Results A total of 605 components in Houttuynia cordata Injection were obtained,of which 10 components were in total.They were 2,4-dimethylheptane,3,7-dimethyldecane,2,6-dimethyldecalin,2,3-dimethyldecahydronaphthalene,5-methyltetradecane,4,6-dimethyldodecane,4-methyldodecane,hexadecane,heptadecane,2,4-bis(1,1-dimethylethyl)phenol;All the components were classified into 10 component groups.The similarity of each component group ranged from 0.934 to 1.000.The effect coefficients of each component group were obtained by nonlinear regression,and the information of component group 3 was the largest(17.67%)by further calculation.Conclusion Based on theoretical guidance of supramolecular“imprinting template”,the components in Houttuynia cordata Injection were classified.The order of sensitization of each component group was obtained by nonlinear regression,and component group 3 may be the key component group causing sensitization.

关 键 词：鱼腥草注射剂 体内 超分子 印迹模板 致敏原 非线性回归 2 6-二甲基癸烷 

分 类 号：R285.5[医药卫生—中药学]