高通量技术筛选的米托蒽醌促进肝癌HepG2细胞凋亡的作用及机制  

Effect of High-Throughput-Screened Mitoxantrone on Apoptosis of HepG2 Cells and Its Mechanism of Action

作　　者：晏毛毛 刘宇华 蔡珊珊 何星星[1] 谢步善[1] YAN Mao-mao;LIU Yu-hua;CAI Shan-shan;HE Xing-xing;XIE Bu-shan(Department of Gastroenterology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第一附属医院消化内科,南昌330006

出　　处：《南昌大学学报（医学版）》2022年第1期20-23,共4页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生健康委科技计划项目(20203103)。

摘　　要：目的探讨高通量技术(HTS)筛选的米托蒽醌(MTX)促进肝癌细胞凋亡的作用及机制。方法将不同浓度的MTX(0、0.5、1、2.5、5、10、20μmol·L^(-1))处理肝癌HepG2细胞24 h,采用MTT法检测HepG2细胞增殖抑制率,采用免疫印迹检测HepG2细胞凋亡标记分子cleaved Caspase-3的表达,采用流式细胞术检测HepG2细胞凋亡率。结果MTX抑制肝癌HepG2细胞增殖和生长,且呈浓度依赖性,其中5、10、20μmol·L^(-1) MTX处理的肝癌HepG2细胞增殖抑制率分别为0.69、0.67、0.43;与10μmol·L^(-1)MTX比较,20μmol·L^(-1) MTX的增殖抑制率显著下降(P<0.001)。与0μmol·L^(-1) MTX处理的肝癌HepG2细胞比较,5、10μmol·L^(-1) MTX轻度上调cleaved Caspase-3的表达,而20μmol·L^(-1) MTX显著上调cleaved Caspase-3的表达(P<0.05)。0μmol·L^(-1) MTX处理的肝癌HepG2细胞凋亡率为9.07%,20μmol·L^(-1) MTX处理的肝癌HepG2细胞凋亡率为21.12%,二者比较差异有统计学意义(P<0.05)。结论HTS筛选出的MTX可抑制肝癌细胞生长增殖,诱导cleaved Caspase-3表达增加,促进肝癌细胞凋亡。Objective To explore the effect of mitoxantrone(MTX)screened by high-throughput screening(HTS)on hepatoma cell apoptosis and its mechanism of action.Methods HepG2 cells were treated with different concentrations of MTX(0.5,1,2.5,5,10 and 20μmol·L^(-1))for 24 hours.Cell proliferation,caspase-3 expression and apoptosis were detected by MTT assay,Western blot and flow cytometry,respectively.Results MTX treatment inhibited the proliferation of HepG2 cells in a concentration-dependent manner.The inhibition rates of cell proliferation were 0.69,0.67 and 0.43 in 5,10 and 20μmol·L^(-1) MTX treatment groups,respectively.The inhibition rate in 20μmol·L^(-1) MTX treatment group was significantly lower than that in 10μmol·L^(-1) MTX treatment group(P<0.001).Compared with the control group,the expression of caspase-3 was slightly increased by challenge with 5 or 10μmol·L^(-1) MTX,but was remarkably up-regulated after treatment with 20μmol·L^(-1) MTX(P<0.05).In addition,the apoptosis rate in 20μmol·L^(-1) MTX treatment group(21.12%)was significantly higher than that in the control group(9.07%)(P<0.05).Conclusion HTS-screened MTX can inhibit proliferation,induce caspase-3 expression,and promote apoptosis in hepatoma cells.

关 键 词：米托蒽醌 高通量筛选技术 肝癌HEPG2细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]