下调微小RNA-425-5p靶向第10号染色体同源缺失性磷酸酶-张力蛋白调控宫颈癌细胞侵袭和迁移的分子机制  

Molecular mechanism of the downregulation of miR-425-5p targeting PTEN to regulate the invasion and migration of cervical cancer cells

作　　者：李敏[1] 赵妍丽[1] 杜国波[1] LI Min;ZHAO Yanli;DU Guobo(Department of Oncology,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)

机构地区：[1]川北医学院附属医院肿瘤科,四川南充637000

出　　处：《安徽医药》2022年第3期523-527,F0003,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究下调微小RNA(miR)-425-5p靶向第10号染色体同源缺失性磷酸酶-张力蛋白(PTEN)调控宫颈癌Caski细胞侵袭和迁移的分子机制。方法本研究起止时间为2018年12月至2019年11月。以宫颈癌Caski细胞为探讨对象,转染miR-425-5p抑制剂(inhibitor),PTENsiRNA和miR-425-5p inhibitor共转染到宫颈癌Caski细胞;MTT法测定细胞增殖,Transwell小室测定细胞侵袭和迁移;在线靶基因预测软件发现PTEN与miR-425-5p可能互为靶向关系,荧光素酶报告系统鉴定靶向关系;蛋白质印迹法(Westernblotting)测定上皮钙黏素(E-cadherin)、神经钙黏素(N-cadherin)蛋白表达。结果转染miR-425-5p inhibitor后的宫颈癌Caski细胞miR-425-5p表达量降低[(0.96±0.15)比(0.32±0.04)],增殖[(0.47±0.05)比(0.23±0.04)]、侵袭[(95.32±7.86)比(63.17±5.22)]及迁移[(140.88±13.94)比(89.64±9.57)]能力降低,E-cadherin表达上调[(0.29±0.05)比(0.65±0.07)],N-cadherin表达下调[(0.59±0.04)比(0.30±0.04)]。miR-425-5p靶向负调控PTEN表达。PTENsiRNA可以逆转下调miR-425-5p对宫颈癌Caski细胞增殖、侵袭和迁移的抑制作用。结论下调miR-425-5p靶向PTEN抑制宫颈癌Caski细胞侵袭和迁移。Objective To investigate the molecular mechanism of the downregulation of microRNA(miR)-425-5p targeting phospha⁃tase and tensin homolog deleted on chromosome ten(PTEN)to regulate the invasion and migration of cervical cancer CaSki cells.Meth⁃ods The study period was from December 2018 to November 2019.Cervical cancer Caski cells were used as the research object,transfected with miR-425-5p inhibitor,and cotransfected with PTEN siRNA and miR-425-5p inhibitor into cervical cancer Caski cells.The MTT assay was used to measure cell proliferation,and a Transwell chamber was used to measure cell invasion and migration.The online target gene prediction software found that PTEN and miR-425-5p may have a targeting relationship with each other,and the lu⁃ciferase reporter system identified the targeting relationship.Western blotting was used to determine the protein expression of epithelial cadherin(E-cadherin)and neural cadherin(N-cadherin).Results The expression of miR-425-5p in cervical cancer Caski cells trans⁃fected with miR-425-5p inhibitor decreased[(0.96±0.15)vs.(0.32±0.04)],proliferation[(0.47±0.05)vs.(0.23±0.04)],invasion[(95.32±7.86)vs.(63.17±5.22)]and migration[(140.88±13.94)vs.(89.64±9.57)]were decreased.The expression of E-cadherin[(0.29±0.05)vs.(0.65±0.07)]was upregulated,while the expression of N-cadherin[(0.59±0.04)vs.(0.30±0.04)]was downregulated.miR-425-5p targets and negatively regulates PTEN expression.PTEN siRNA can reverse the inhibitory effect of downregulation of miR-425-5p on the pro⁃liferation,invasion and migration of cervical cancer CaSki cells.Conclusion Downregulation of miR-425-5p targeting PTEN inhibits the invasion and migration of cervical cancer CaSki cells.

关 键 词：子宫肿瘤 CASKI细胞 钙黏着糖蛋白类 微小RNA-425-5p 第10号染色体同源缺失性磷酸酶-张力蛋白 转移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]