A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats  

作　　者：Xuan-Yu Fang Da-Wei Zhao Chao Zhang Hong-Fei Ge Xu-Yang Zhang Feng-Chun Zhao Yi-Bin Jiang Hua Feng Rong Hu 

机构地区：[1]Department of Neurosurgery and Key Laboratory of Neurotrauma,Southwest Hospital,Army Medical University,Chongqing,China

出　　处：《Neural Regeneration Research》2022年第11期2512-2517,共6页中国神经再生研究（英文版）

基　　金：financially the Project,No.16QNP101;the Natural Science Foundation for Outstanding Youth of Chongqing,China No.cstc2019jcyjjqX0030(both to RH)。

摘　　要：Brain lesions can cause neural stem cells to activate,proliferate,diffe rentiate,and migrate to the injured area.However,after traumatic brain injury,brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells;the resulting reduction in the number of neural stem cells impedes effective repair of the injured area.Melatonin can promote the survival,proliferation,and differentiation of neural stem cells under adverse conditions such as oxidative stress or hypoxia that can occur after traumatic brain injury.Therefore,we investigated the therapeutic effects of melatonin combined with neural stem cells on traumatic brain injury in rats.First,in vitro studies confirmed that melatonin promoted the survival of neural stem cells deprived of oxygen and glucose.Then,we established a three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells and then used it to treat traumatic brain injury in rats.We found that treatment with the Matrigel system containing melatonin and neural stem cells decreased brain lesion volume,increased the number of surviving neuro ns,and improved recove ry of neurological function compared with treatment with Matrigel alone,neural stem cells alone,Matrigel and neural stem cells combined,and Matrigel and melatonin combined.Our findings suggest that the three-dimensional Matrigelbased transplantation system containing melatonin and neural stem cells is a potential treatment for traumatic brain injury.

关 键 词：cell therapy magnetic resonance imaging MATRIGEL MELATONIN neural stem cells neurological function recovery three-dimensional transplantation traumatic brain injury 

分 类 号：R651.15[医药卫生—外科学]