Human umbilical cord-derived mesenchymal stem cells promote repair of neonatal brain injury caused by hypoxia/ischemia in rats  

作　　者：Yang Jiao Yue-Tong Sun Nai-Fei Chen Li-Na Zhou Xin Guan Jia-Yi Wang Wen-Juan Wei Chao Han Xiao-Lei Jiang Ya-Chen Wang Wei Zou Jing Liu 

机构地区：[1]Stem Cell Clinical Research Center,National Joint Engineering Laboratory,Regenerative Medicine Center,The First Affiliated Hospital of Dalian Medical University,Dalian,Liaoning Province,China [2]Dalian Innovation Institute of Stem Cells and Precision Medicine,Dalian,Liaoning Province,China [3]Department of Neurology,The First Affiliated Hospital of Dalian Medical University,Dalian,Liaoning Province,China [4]College of Life Science,Liaoning Normal University,Dalian,Liaoning Province,China

出　　处：《Neural Regeneration Research》2022年第11期2518-2525,共8页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,No.81471308(to JL);Stem cell Clinical Research Registry Program,No.CMR-20161129-1003(to JL);Liaoning Province Excellent Talent Program Project of China,No.XLYC1902031(to JL);Dalian Innovation Fund of China,No.2018J11CY025(to JL);National Defense Science and Technology New Special Zone Contract,No.19-163-00-kx-003-001-01(to JL)。

摘　　要：Administration of human umbilical cord-derived mesenchymal stem cells(hUC-MSCs)is believed to be an effective method for treating neurodevelopmental disorde rs.In this study,we investigated the possibility of hUC-MSCs treatment of neonatal hypoxic/ischemic brain injury associated with maternal immune activation and the underlying mechanism.We established neonatal rat models of hypoxic/ischemic brain injury by exposing pregnant rats to lipopolysaccharide on day 16 or 17 of pregnancy.Rat offspring were intranasally administe red hUC-MSCs on postnatal day 14.We found that polypyrimidine tract-binding protein-1(PTBP-1)participated in the regulation of lipopolysaccharide-induced maternal immune activation,which led to neonatal hypoxic/ischemic brain injury.Intranasal delive ry of hUC-MSCs inhibited PTBP-1 expression,alleviated neonatal brain injury-related inflammation,and regulated the number and function of glial fibrillary acidic protein-positive astrocytes,there by promoting plastic regeneration of neurons and im p roving brain function.These findings suggest that hUC-MSCs can effectively promote the repair of neonatal hypoxic/ischemic brain injury related to maternal immune activation through inhibition of PTBP-1 expression and astrocyte activation.

关 键 词：developmental brain disease model disease-associated astrocytes intranasal administration LIPOPOLYSACCHARIDE maternal immune activation neonatal brain injury neuroplasticity repair polypyrimidine tract-binding protein-1 stem cell therapy umbilical cord-derived mesenchymal stem cells 

分 类 号：R742[医药卫生—神经病学与精神病学]