PPAR-γ2单核苷酸多态性与糖化血红蛋白水平的交互作用和糖尿病性牙周炎易感性的关系  

Interaction between PPAR-γ2 single nucleotide polymorphism and glycosylated hemoglobin level and susceptibility to diabetic periodontitis

作　　者：刘钦赞 庞真贞 刘莉芳[1] LIU Qin-zan;PANG Zhen-zhen;LIU Li-fang(Department of Stomatology,Btwding First Central Hospital,Dented Doctor Hospital of Baoding,Baoding 071000,Hebei,China)

机构地区：[1]保定市第一中心医院口腔一科,保定天使牙博士口腔医院,河北保定071000

出　　处：《生物医学工程与临床》2022年第1期91-98,共8页Biomedical Engineering and Clinical Medicine

摘　　要：目的探讨过氧化物酶体增殖物激活受体-γ2基因(PPAR-γ2)单核苷酸多态性(SNP)与糖化血红蛋白(HbA1c)水平的交互作用和糖尿病性牙周炎(DP)易感性的关系。方法选择2017年8月至2020年4月于保定市第一中心医院就诊的DP患者259例,其中男性138例,女性121例;年龄28~55岁,平均年龄42.98岁;体质量指数(BMI)22.42~32.13 kg/m^(2),平均BMI 27.25 kg/m^(2);吸烟64例,高血压史78例。2型糖尿病(T2DM)患者257例,其中男性124例,女性133例;年龄31~59岁,平均年龄43.31岁;BMI 22.74~29.88 kg/m^(2),平均BMI 25.87 kg/m^(2);吸烟56例,高血压史85例。选择同期接受健康体检者180例作为对照组,其中男性94例,女性86例;年龄28~60岁,平均年龄43.30岁;BMI 21.13~27.03 kg/m^(2),平均BMI 22.57 kg/m^(2);吸烟30例,高血压史69例。采用全自动生化仪检测T2DM患者HbA1c水平;采用双脱氧末端终止法检测PPAR-γ2 Pro12Ala SNP;采用多因素Logistic回归模型分析DP发生的危险因素,估算PPAR-γ2Pro12Ala SNP和HbA1c水平与DP发病风险的调整比值比(OR)和95%可信区间(95%CI);分析Pro12Ala SNP与HbA1c水平的交互作用。结果对照组、T2DM组及DP组患者HbA1c水平分别为5.12%±0.97%、7.95%±1.21%和9.12%±1.35%,组间比较差异具有统计学意义(P <0.05)。基因分型后可得纯合子Pro/Pro(P/P)型片段为224 bp、43 bp,杂合突变Pro/Ala(P/A)型片段为276 bp、224 bp、43 bp,纯合突变Ala/Ala(A/A)型片段为267 bp。Pro12Ala(PA)、Pro12Ala(AA)基因型T2DM患者患DP的风险均增加(OR=2.529,95%CI为1.637~3.498;OR=2.594,95%CI为1.645~3.856)。6.0%≤HbA1c <8.0%和HbA1c≥8.0%的T2DM患者患DP的风险明显增加(OR=2.046,95%CI为1.358~2.952;OR=3.105,95%CI为2.049~4.861),且HbA1c≥8.0%的T2DM患者患DP的风险又明显高于6.0%≤HbA1c <8.0%的T2DM患者(χ^(2)=6.087,P <0.01);HbA1c水平与Pro12Ala(PA)、Pro12Ala(AA)均存在正向交互作用(γ> 1)。结论携带Pro12Ala(PA)、Pro12Ala(AA)基因型的个体属DP高危人群,这些基因型与HbA1c水�Objective To investigate the relationship between peroxisome proliferator activated receptor-γ2 gene(PPAR-γ2) single nucleotide polymorphism and glycosylated hemoglobin( HbA1 c) level and susceptibility to diabetic periodontitis( DP).Method From August 2017 to April 2020, a total of 259 DP patients were enrolled, which included 138 males and 121 females, aged 28-55 years old with mean age of 42.98 years old;body mass index(BMI) was 22.42-32.13 kg/m^(2) with mean BMI of27.25 kg/m^(2);64 cases with smoking history and 78 with hypertension. The 257 patients with type 2 diabetes mellitus(T2 DM)were set as T2 DM group, which included 124 males and 133 females, aged 31-59 years old with mean age of 43.31 years old;BMI was 22.74-29.88 kg/m^(2) with mean BMI of 25.87 kg/m^(2);56 cases with smoking history and 85 with hypertension.Meanwhile, 180 healthy physical examination subjects were set as control group, which included 94 males and 86 females;aged 28-60 years old with mean age of 43.30 years old;BMI was 21.13-27.03 kg/m^(2) with mean BMI of 22.57 kg/m^(2);30 subjects with smoking history and 69 with hypertension. The multivariate Logistic regression model was used to analyze risk factors of DP, and estimate adjusted odds ratio(OR) and 95 % confidence interval(95 % CI) of PPAR-γ2 Pro12 Ala SNP and HbA1 c levels to DP risk. The interaction between Pro12 Ala SNP and HbA1 c level was analyzed. Results The levels of HbA1 c in control group, T2 DM group and DP group were 5.12 % ± 0.97 %, 7.95 % ± 1.21 % and 9.12 % ± 1.35 %, respectively, and the differences between the groups were statistically significant(P < 0.05). After genotyping, homozygous Pro/Pro(P/P)fragment was 224 bp and 43 bp, heterozygous mutant Pro/Ala(P/A) fragment was 276 bp, 224 bp and 43 bp, and homozygous mutant Ala/Ala(A/A) fragment was 267 bp. Patients with Pro12 Ala(PA) and Pro12 Ala(AA) genotype T2 DM had an increased risk to DP(OR = 2.529, 95 % CI 1.637-3.498;OR = 2.594, 95 % CI 1.645-3.856). The risk of DP in T2 DM patients with 6.0 % ≤ HbA1

关 键 词：过氧化物酶体增殖物激活受体-γ2(PPAR-γ2) 糖化血红蛋白(HbA1c) 糖尿病性牙周炎 单核苷酸多态性 交互作用 

分 类 号：R781.42[医药卫生—口腔医学] R587.2[医药卫生—临床医学]