多能干细胞体外分化为类神经管模型的研究进展  

Development of neural tube-like models derived from pluripotent stem cells in vitro

作　　者：张小佐 霍海芹 谭建新[1,2] 张芳 冯浩洋[1,2] 许争峰 Zhang Xiaozuo;Huo Haiqin;Tan Jianxin;Zhang Fang;Feng Haoyang;Xu Zhengfeng(Department of Prenatal Diagnosis,the Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University,Nanjing 210000,China;Department of Prenatal Diagnosis,Nanjing Maternity and Child Health Care Hospital,Nanjing 210000,China)

机构地区：[1]南京医科大学附属妇产医院产前诊断中心,210000 [2]南京市妇幼保健院产前诊断中心,210000

出　　处：《中华细胞与干细胞杂志（电子版）》2022年第1期45-50,共6页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家重点研发计划课题(2018YFC1002402);国家自然科学基金(82001212)。

摘　　要：近年来多能干细胞(PSCs)的体外培养与分化技术发展迅速,并广泛应用于再生医学和发育生物学等领域。PSCs能够在体外神经诱导的条件下分化为类神经管模型,这为探索体内早期神经发育与中枢神经系统发育疾病的形成机制提供了全新的实验平台。本文总结了近年来应用小鼠和人PSCs建立体外类神经管模型的研究进展,其中体外模型主要包括在不同培养体系下诱导获得的二维(2D)与三维(3D)类神经管模型,并针对早期类神经管模型在神经系统发育性疾病机制研究中的前景和挑战作进一步探讨,同时为疾病预防和治疗提供新的思路。The approaches for culture and differentiation of pluripotent stem cells in vitro have developed rapidly, and have widely applied in the fields of regenerative medicine and developmental biology. Neural tube-like models can be established from pluripotent stem cells in vitro under neural induction, which provide a new experimental platform for exploring early neural development in vivo, and it is also benefit for exploring the mechanisms of central neural system developmental diseases. Herein, we summarized the progress of neural tube-like models derived from mouse and human pluripotent stem cells in vitro, so as to find the prospects and challenges of neural tube-like models in investigating the mechanism of neural system developmental diseases, and also can provide novel insights into for disease prevention and treatment.

关 键 词：多能干细胞 类器官 神经管 神经诱导 

分 类 号：R741[医药卫生—神经病学与精神病学]