扁茎黄芪转录组测序及生物信息学分析  

Transcriptome Sequencing and Bioinformatics Analysis of Astragalus complanatus

作　　者：武悦 陈阳[2] 王星哲 单飞彪 张勇[2] 孙鸿举[1] WU Yue;CHEN Yang;WANG Xingzhe;SHAN Feibiao;ZHANG Yong;SUN Hongju(School of Life Sciences,Inner Mongolia University,Hohhot 010020,China;Bayannur Academy of Agricultural and Animal Sciences,Linhe 015000,China)

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010020 [2]巴彦淖尔市农牧业科学研究院,内蒙古临河015000

出　　处：《华北农学报》2022年第1期42-49,共8页Acta Agriculturae Boreali-Sinica

基　　金：内蒙古科技重大专项(2019ZD009);内蒙古自治区应用技术研究与开发资金资助项目(5213322)。

摘　　要：为获得扁茎黄芪转录组信息及功能基因表达特征,以扁茎黄芪的幼苗叶片为材料,利用Illumina HiSeq平台进行转录组测序及生物信息学系统分析。结果显示,共获得扁茎黄芪Unigene 19280条,总长23472470 bp,其中GC含量达42.74%,测序质量和组装效果较好。通过Blast分析后,分别有12541,10120,9412,8953,7494,5052条Unigene在Nr、Swiss-Prot、GO、KEGG、KOG、COG数据库获得注释。注释到的扁茎黄芪Unigene同源物种以豆科植物为主,尤其是与其同属蝶形花亚科的鹰嘴豆、蒺藜苜蓿和相思子匹配度较高,分别占31.49%,14.50%,11.65%;扁茎黄芪注释Unigene涉及3个GO类别,354条KEGG代谢通路和25个KOG功能分类,其中富集最多的类别分别为代谢过程、一般功能预测和嘌呤代谢,说明扁茎黄芪幼苗期的叶片细胞具有活跃的新陈代谢活动,基因表达丰富。另外,扁茎黄芪Unigene在KEGG数据库中的感染性疾病类别获得较多注释,表明其植株部位也可能具有药用价值。利用MISA软件挖掘到5849个SSR位点,SSR出现频率30.34%。扁茎黄芪基因组内SSR位点丰富、类型多样化,单碱基至六碱基重复全部出现,其中单碱基丰度最高,占40.56%,以A/T、AG/CT和AAG/CTT为优势基元。In order to obtain transcriptome information and functional genes expression characteristics of Astragalus complanatus,the leaves of A.complanatus were used as material,the high-throughput transcriptome sequencing and bioinformatics analysis were performed using Illumina HiSeq platform.A total of 19280 Unigenes were assembled with total sequence length of 23472470 bp,of which the GC content was 42.74%,indicating a high quality of sequencing and assembly.Blast analysis showed 12541,10120,9412,8953,7494,5052 Unigenes got annotation in Nr,Swiss-Prot,GO,KEGG,KOG and COG databases,respectively.The annotated Unigenes of A.complanatus were mainly homologuous to leguminous plants,in particular,highly matched to the subfamily Pteriformis plant Cicer arietinum,Medicago truncatula and Abrus precatorius with a respective percentage of 31.49%,14.50%and 11.65%.Annotated Unigenes were divided into 3 GO classifications,354 KEGG metabolic pathways and 25 KOG function categories.The most abundant categories in each database were metabolic process,general function prediction only and purine metabolism,respectively.These results showed that the leaf cells of Astragalus membranaceus had active metabolism and abundant gene expression in the seedling stage.Additionally,a lot of Unigenes were annotated to the infectious disease category in KEGG database,suggesting a potential medicinal value of the plant part of Astragalus membranaceus.A total of 5849 simple sequence repeats(SSRs)were identified by MISA software,with an occurrence frequency of 30.34%.The SSRs in the genome of Astragalus membranaceus were abundant and diversified,containing single base to six base repeats,among which the mono-nucleotide SSRs had the largest number with a frequency of 40.56%.The main types of SSR motifs involved A/T,AG/CT and AAG/CTT.

关 键 词：扁茎黄芪 转录组 生物信息学 UNIGENE 分子标记 

分 类 号：Q78[生物学—分子生物学] S567.03[农业科学—中草药栽培]