抗抑郁药对海马星形胶质细胞缝隙连接蛋白表达和通道功能的影响  

Influence of antidepressants on connexin expression and gap junction function of hippocampal astrocytes

作　　者：梁沛彰 颜茹芳 张永华 黄焕森[1] 汪灵芝[1] Liang Peizhang;Yan Rufang;Zhang Yonghua;Huang Huansen;Wang Lingzhi(Department of Anesthesiology,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China)

机构地区：[1]广州医科大学附属第二医院麻醉科,广州510260

出　　处：《新医学》2022年第3期188-192,共5页Journal of New Medicine

基　　金：广东省自然科学基金(2020A151501071);广州医科大学高水平大学学术骨干培育项目;广州市基础研究计划市校(院)联合资助基础与应用基础研究项目。

摘　　要：目的探讨3种不同机制抗抑郁药对海马星形胶质细胞缝隙连接蛋白和通道功能的影响。方法CCK-8实验检测不同机制抗抑郁药对海马星形胶质细胞的细胞毒性,采用蛋白免疫印迹法和细胞接种荧光示踪法测定3种不同机制抗抑郁药对由缝隙连接蛋白43(Cx43)组成的通道功能和Cx43蛋白表达的影响。结果CCK-8实验显示,25.00μmol/L氟西汀、0.10μmol/L阿米替林和0.20 nmol/L文拉法辛对海马星形胶质细胞无细胞毒性(P均>0.05);25.00μmol/L氟西汀、0.10μmol/L阿米替林和0.20 nmol/L文拉法辛能抑制海马星形胶质细胞缝隙连接的荧光传递功能(P均<0.05),但3种抗抑郁药均不影响海马星形胶质细胞Cx43蛋白的表达(P均>0.05)。结论抗抑郁药能够显著降低海马星形胶质细胞Cx43组成的通道功能。Objective To evaluate the effects of three antidepressants with different mechanisms on connexin expression and channel function of hippocampal astrocytes.Methods The cytotoxicity of antidepressants with different mechanisms on hippocampal astrocytes was assessed by CCK-8 assay.The effects of three antidepressants with different mechanisms on gap junction function and the expression level of connexin 43 protein(Cx43)were determined by cell inoculation fluorescence tracing and western blot.Results CCK-8 assay showed that 25.00μmol/L fluoxetine,0.10μmol/L amitriptyline and 0.20 nmol/L venlafaxine had no cytotoxicity on hippocampal astrocytes(all P>0.05).In addition,25.00μmol/L fluoxetine,0.10μmol/L amitriptyline and 0.20 nmol/L venlafaxine could significantly inhibit the fluorescence transfer function of gap junction in hippocampal astrocytes(all P<0.05),whereas three antidepressants exerted on effects upon the expression of Cx43 in hippocampal astrocytes(all P>0.05).Conclusion Antidepressants can significantly reduce the gap junction function composed of Cx43 in hippocampal astrocytes.

关 键 词：细胞缝隙连接 星形胶质细胞 海马 抗抑郁药 缝隙连接蛋白43 

分 类 号：R749.4[医药卫生—神经病学与精神病学]