意向性运动干预影响局部脑缺血大鼠GLUA2和N-cadherin表达的影响  

Effects of Willed Movement on the Expression of GLUA2 and N-cadherin in Rats with Focal Cerebral Ischemia

作　　者：蒋雯 梅松[1] 李海江[1] 蒋国梁[1] 黄丹 JIANG Wen;MEI Song;LI Haijiang;JIANG Guoliang;HUANG Dan(Dept.of Neurology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Dept.of Renal Endocrinology,Kunming Tongren Hospital,Kunming Yunnan 650228,China)

机构地区：[1]昆明医科大学第一附属医院神经内科,云南昆明650032 [2]昆明同仁医院肾脏内分泌科,云南昆明650228

出　　处：《昆明医科大学学报》2022年第3期21-26,共6页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202001AY07001-186);云南省神经系统疾病临床医学研究中心应用研究基金资助项目(202002AA100204);云南省神经系统疾病诊疗中心应用基础研究基金资助项目(ZX2019-03-05)。

摘　　要：目的研究意向性运动干预治疗对大脑局部缺血大鼠行为学及梗死灶周围组织中GluA2和Ncadherin表达的影响。方法利用鱼线栓堵法将大鼠制成大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型共144只,随机分成模型(MCAO)组、生活环境变化(environment modification,EM)组、普通康复训练(control rehabilitstion,CR)组和意向性运动干预(willed movement,WM)组,每组根据术后时间划分为7 d、15 d、30 d 3个亚组。对各组研究对象的行为变化进行观察分析,通过免疫组化技术研究脑梗死灶周围组织中GluA2和N-cadherin蛋白水平。结果再灌7 d、15 d、30 d,意向性运动干预组的攀爬频率显著高于EM及CR组(P<0.01);再灌30 d,和MCAO组相比,意向性运动干预组的神经功能缺损评分更低(P<0.05);而其缺血半暗带区域GLUA2、N-cadherin蛋白表达量都更高(P<0.05)。结论对于大脑局部缺血所导致的神经功能障碍,意向性运动干预治疗可促进其恢复,这可能是因为该运动可激活缺血半暗带中GLUA2和N-cadherin的表达,提高突触可塑性。Objective To explore effect of the Willed movement on behaviours of rats with focal cerebral ischemia and the expression of GluA2 and N-cadherin in the brain tissue around ischemia.Methods We selected clean-level healthy male SD rats,and prepared 144 middle cerebral artery occlusion(MCAO)models by thread occlusion method,and randomly divided them into model(MCAO)group,environment modification(EM)group,control rehabilitstion(CR)group and Willed movement(WM)group,each group was divided into four subgroups of7,15,and 30 days according to the postoperative time.The behavioral changes of rats in each group were dynamically observed,and the expression changes of GluA2 and N-cadherin protein in the brain tissue around the ischemic area were observed by immunohistochemical staining.Results On 7,15,and 30 days after reperfusion,the climbing frequency of rats which were treated by willed movement was obviously higher than EM and CR group(P<0.01).After 30 days of reperfusion,the neurological deficits score of the intentional exercise intervention group was lower than MCAO rats(P<0.05).Compare with MCAO rats,the expression levels of GLUA2 and N-cadherin were higher in ischemic penumbra(P<0.05).Conclusions Willed movement can promote the recovery of damaged nerve function after focal cerebral ischemia,which may be related to up-regulating the expression of GLUA2 and N-cadherin in the brain tissue around the ischemic focus and enhancing synaptic plasticity.

关 键 词：局灶性脑缺血 意向性运动 突触可塑性 GLUA2 N-CADHERIN 

分 类 号：R741.02[医药卫生—神经病学与精神病学]