裙带菜岩藻聚糖硫酸酯酸解工艺优化及降解产物对巨噬细胞的抗炎活性  

Optimization of preparation process of fucoidan from sea mustard Undaria pinnatifida Suringar by acid hydrolysis and anti-inflammatory activity of degradation products on macrophages

作　　者：李晓旭 刘舒 洪滨 刘文博 任丹丹 汪秋宽 何云海 洪瑞晨 付开艳 宋悦凡 LI Xiaoxu;LIU Shu;HONG Bin;LIU Wenbo;REN Dandan;WANG Qiukuan;HE Yunhai;HONG Ruichen;FU Kaiyan;SONG Yuefan(College of Food Science and Engineering, National R&D Branch Center For Seaweed Processing, Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China;Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China;Dalian Customs Technology Center, Dalian 116014, China)

机构地区：[1]大连海洋大学食品科学与工程学院,国家海藻加工技术研发分中心,辽宁省水产品加工及综合利用重点实验室,辽宁大连116023 [2]海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [3]大连海关技术中心,辽宁大连116014

出　　处：《大连海洋大学学报》2022年第1期166-173,共8页Journal of Dalian Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”项目(2018YFD0901104);辽宁省教育厅科研项目(JL202009,JL201909);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-50);大连市青年科技之星项目(2020RQ074)。

摘　　要：为了有效降解裙带菜Undaria pinnatifida Suringar岩藻聚糖硫酸酯,并探究其降解产物的抗炎活性,以复合酶解法提取的裙带菜岩藻聚糖硫酸酯(fucoidan)为原料,利用酸解法制备裙带菜岩藻聚糖硫酸酯降解产物,采用薄层色谱法(TLC)检测降解产物聚合度,优化酸解工艺条件,利用膜分离技术获得不同分子量段的降解产物,并检测了各段降解产物对小鼠巨噬细胞的抗炎活性。结果表明:通过薄层色谱检测,确定酸解温度95℃、pH 2、水解4 h为酸解裙带菜岩藻聚糖硫酸酯的优化条件;膜分离处理后可得到F1(相对分子质量400~1000)、F2(1000~3500)、F3(3500~5000)、F4(5000~10000)、F5(10000以上)5种不同分子量段的岩藻聚糖硫酸酯降解产物,5种降解产物均能减少RAW264.7细胞NO、1L-1β、1L-6、1L-10和TNF-α的释放量;低分子量段组分(F1、F2)抗炎效果最为显著,其中组分F1抗炎效果最好。研究表明,经优化酸解工艺制备的裙带菜岩藻聚糖硫酸酯降解产物具有一定的抗炎效果,且分子量越低的降解产物抗炎效果越好,裙带菜岩藻聚糖硫酸酯寡糖比多糖具有更好的抗炎活性。In order to effectively degrade fucoidan from sea mustard Undaria pinnatifida and explore the anti-inflammatory activity of its degradation products,degradation products of fucoidan extracted from sea mustard by complex enzymatic hydrolysis was prepared by acid hydrolysis,and the hydrolysis process conditions were optimized via hydrolysis temperature,period,and pH values.The polymerization degree of the degradation products was detected by thin-layer chromatography.Degradation products of different molecular weight segments were obtained by membrane separation technology,and the anti-inflammatory activity of the degradation products on macrophages in mice was detected.The results showed that fucoidan degradation products of F1,F2,F3,F4 and F5 with different molecular weight segments were obtained by thin layer chromatography(TLC)under the optimal conditions of 95℃and pH 2 for 4 h.All the five degradation products led to reduce the release of NO,1L-1β,1L-6,1L-10 and TNF-αin RAW264.7 cells,with the most significant anti-inflammatory effect of the low molecular weight fractions(F1,F2),especially the fucoidan oligosaccharide F1.The findings indicated that the degradation products of fucoidan prepared by the optimized acidolysis process had a certain anti-inflammatory effect,the lower the molecular weight of the degradation products was,the better the anti-inflammatory effect was.There was better anti-inflammatory effect in low molecular weight fucoidan oligosaccharide than that in the large molecular weight fucoidan.

关 键 词：裙带菜 岩藻聚糖硫酸酯寡糖 酸解法 抗炎活性 

分 类 号：S985.4[农业科学—捕捞与储运] TQ464[农业科学—水产科学]