胆囊收缩素对小肠I细胞及胰腺细胞和小鼠胰腺消化酶储备的影响  

Effects of cholecystokinin on the intestinal I cells,pancreatic cells and pancreatic digestive enzyme reserve in mice

作　　者：徐晓晴 邱帅 陈喜娟 胡立娟 李秋菊 张大鹏[2] 王丰[2] XU Xiao-qing;QIU Shuai;CHEN Xi-juan;HU Li-juan;LI Qiu-ju;ZHANG Da-peng;WANG Feng(Graduate School,Tianjin Medical University,Tianjin 300070,China;The Laboratory of Acute Abdomen Disease Associated Organ Injury and Repair,Nankai Hospital Affiliated to Nankai University,Tianjin 300100,China)

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津市南开医院中西医结合急腹症研究所,天津300100

出　　处：《天津医科大学学报》2022年第2期156-159,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金(81572318)。

摘　　要：目的:研究胰胆转流(PBD)术造成的高胆囊收缩素(CCK)血症对小鼠小肠I细胞群落及胰腺细胞和胰腺消化酶含量的影响。方法:40只雄性Balb/C裸鼠随机分为PBD组及假手术(SO)组,每组20只,分别实施PBD术和假手术。20 d后称重胰腺重量,并用酶联免疫吸附法测定血浆CCK,用免疫组化法在十二指肠中测定I细胞群落,在胰腺中检测CCK受体和增殖细胞核抗原,以酶联免疫吸附法测定胰腺组织中胰蛋白酶原、脂肪酶、淀粉酶的含量。结果:PBD组血浆CCK水平高于SO组(t=16.59,P<0.01),胰腺重量也较SO组增加(t=2.51,P<0.05);且PBD组十二指肠内的I细胞多于SO组(t=5.04,P<0.01)。进一步的胰腺检测显示,PBD组CCK受体表达增强(t=5.08,P<0.01)、增殖细胞核抗原增多(t=8.05,P<0.01);比较两组胰腺外分泌酶的组成,PBD组胰腺中的胰蛋白酶原(t=4.31,P<0.01)、脂肪酶(t=2.15,P<0.05)的浓度增加;胰淀粉酶浓度虽然较SO组有增加的趋势,但差异无统计学意义(t=0.60,P=0.56);PBD组全胰蛋白酶含量(t=2.49,P<0.05)、脂肪酶含量(t=2.02,P<0.05)、淀粉酶含量(t=2.53,P<0.05)均较SO组增高。结论:CCK可刺激胰腺发育,使小肠I细胞增生,选择性地增加胰腺中的胰蛋白酶原和胰脂酶储备。Objective:To study the effects of hypercholecystokin(CCK)inemia induced by pancreaticobiliary(PBD)on the proliferation of small intestinal I cell and pancreatic cells and the composition of pancreatic exocrine enzymes in mice.Methods:Forty male mice were randomly divided into PBD group and sham operation(SO)group,with 20 in each group.After 20 days,the weight of pancreas was weighed,and plasma CCK was determined by ELISA.The I cell that expressed CCK in the duodenum and the cells that expressed the CCK receptor(CCKR)and the proliferating cell nuclear antigen(PCNA)in the pancreas were determined by immunohistochemistry.The trypsinogen,lipase,and amylase were determined in the pancreas,using commercial kits.Results:The PBD group had higher plasma CCK levels,compared with SO group(t=16.59,P<0.01).The pancreas was bigger in the PBD group than in the SO group(t=2.51,P<0.05).When I cells were quantified in the duodenum,the PBD group showed significant increase,compare to the SO group(t=5.04,P<0.01).The PBD group had greater expression of CCKR(t=5.08,P<0.01)and also had more proliferating cells(PCNA positive)(t=8.05,P<0.01),than the SO group.Further,when the composition of pancreatic exocrine enzymes was compared between the PBD and SO group,the concentration of pancreatic trypsinogen(t=4.31,P<0.01)and lipase(t=2.15,P<0.05)increased in PBD group.Although the concentration of pancreatic amylase increased compared with SO group,there was no statistical significance(t=0.60,P=0.56).The trypsin content(t=2.49,P<0.05),the lipase content(t=2.02,P<0.05)and the amylase content(t=2.53,P<0.05)in PBD group were higher than those in SO group.Conclusion:CCK stimulates pancreatic growth and also increases I cells population in small intestines and selectively increase the reserve of trypsinogen and lipase in pancreas.

关 键 词：胰胆转流术 小鼠 胰腺 胆囊收缩素 分泌 酶学 细胞增殖 I细胞 

分 类 号：R57[医药卫生—消化系统]