亚硒酸钠对帕金森病大鼠黑质形态学及纹状体谷氨酸含量的影响及机制研究  

Effect of Sodium Selenite on the Morphology of the Substantia Nigra and the Content of Glutamate in the Striatum of Rats with Parkinson's Disease

作　　者：范凯[1] FAN Kai(Northern Hospital of Xi'an City,Shaanxi Province,Xi'an 710032)

机构地区：[1]陕西省西安市北方医院,陕西西安710032

出　　处：《医学临床研究》2022年第2期236-239,243,共5页Journal of Clinical Research

摘　　要：【目的】探讨亚硒酸钠对帕金森病(PD)大鼠黑质形态学及纹状体谷氨酸含量的影响及相关机制。【方法】注射6-羟基多巴胺(6-OHDA)建立PD大鼠模型,将20只建模成功大鼠随机分为模型组、实验组,每组10只。另取10只大鼠仅注射生理盐水,设为空白对照组。注射结束后15 d,实验组灌胃亚硒酸钠100 mg/kg,空白对照组、模型组灌胃等体积生理盐水。每天1次,连续30 d。采用T-迷宫实验检测大鼠认知功能;采用高效液相色谱法(HPLC)检测纹状体谷氨酸含量;采用免疫印迹法检测黑质c-Jun氨基末端激酶(JNK)、p-JNK蛋白相对表达量。【结果】空白对照组、模型组、实验组T-迷宫实验正确率分别为(94.44±5.27)%、(50.00±6.39)%、(72.22±8.42)%,纹状体谷氨酸含量分别为(15.96±2.79)μg/mL、(31.87±4.21)μg/mL、(23.34±3.92)μg/mL,黑质p-JNK/JNK分别为0.19±0.02、0.78±0.08、0.27±0.03。空白对照组的T-迷宫实验正确率、纹状体谷氨酸含量、黑质p-JNK/JNK与模型组比较,模型组与实验组比较,差异均有统计学意义(P<0.05)。【结论】亚硒酸钠可改善PD大鼠认知功能、黑质神经元形态,保护黑质多巴胺能神经元功能,减少纹状体谷氨酸含量,其机制可能是通过抑制JNK信号通路来实现。【Objective】To observe the effect of sodium selenite on the morphology of the substantia nigra and the content of glutamate in the striatum of Parkinson's disease(PD)rats,and to explore the related mechanisms.【Methods】A PD rat model was established by injecting 6-Hydroxydopamine(6-OHDA).Twenty successfully modeled rats were randomly divided into the model group and the experimental group,with 10 rats in each group.Ten rats were injected with saline only and set as the control group.Fifteen days after the injection,the experimental group were given sodium selenite 100 mg/kg,while the control group and model group were given an equal volume of normal saline,once a day for 30 consecutive days.The cognitive function was detected by T-maze experiment.The content of glutamate in the striatum were detected by high performance liquid chromatography(HPLC).The relative expression of substantia nigra c-Jun N-terminal kinase(JNK)and p-JNK protein were detected by Western blotting.【Results】The accuracy rates of the T-maze experiment in the control group,the model group and the experimental group were(94.44±5.27)%,(50.00±6.39)%and(72.22±8.42)%,respectively.Of which,the content of glutamate in the striatum were(15.96±2.79)μg/mL,(31.87±4.21)μg/mL and(23.34±3.92)μg/mL.The substantia nigra p-JNK/JNK in the control group,the model group and the experimental group were 0.19±0.02,0.78±0.08 and 0.27±0.03,respectively.The differences of the correct rate of T-maze experiment,striatal glutamate content,substantia nigra p-JNK/JNK ratio between the model group and the control group were statistically significant(P<0.05).They were also significant differences between the model group and the experimental group(P<0.05).【Conclusion】Sodium selenite can improve the cognitive function of PD rats in terms of the morphology of substantia nigra neurons,protect the function of substantia nigra dopaminergic neurons,and reduce the content of striatal glutamate,which may be achieved by inhibiting the JNK signaling pathway.

关 键 词：帕金森病 亚硒酸钠/药理学 纹状体 疾病模型 动物 大鼠 

分 类 号：R741.02[医药卫生—神经病学与精神病学]