慢性间歇性低氧暴露对比目鱼肌和胫骨前肌影响的差异  

Effect of chronic intermittent hypoxia exposure:differences on soleus muscle and musculi tibialis anterior

作　　者：付鹏宇 贾杰 龚丽景 FU Pengyu;JIA Jie;GONG Lijing(Department of Physical Education,Northwestern Polytechnical University,Xi’an,Shaanxi Province,710072;School of Sport Science,Beijing Sport University,Beijing,100084,China;Key Laboratory of Physical Fitness and Exercise of Ministry of Education,Beijing Sport University,Beijing,100084,China)

机构地区：[1]西北工业大学体育部,西安710072 [2]北京体育大学运动人体科学学院,北京100084 [3]北京体育大学运动与体质健康教育部重点实验室,北京100084

出　　处：《陆军军医大学学报》2022年第5期432-440,共9页Journal of Army Medical University

基　　金：中央高校基本科研业务费专项资金(D5000210831);中央高校建设世界一流大学(学科)和特色发展引导专项基金(21GH031119)。

摘　　要：目的对比慢性间歇性低氧暴露对不同纤维类型肌肉的影响及其可能机制。方法SD大鼠16只,分为常氧对照组(C,n=8),置于常氧环境中;慢性间歇性低氧组(H,n=8),置于氧浓度为12.4%的低氧房中,每天暴露8 h,共4周。干预期间称量大鼠体质量,干预后测试抓力和瘦体质量,检测比目鱼肌(soleus muscle,SOL)和胫骨前肌(musculi tibialis anterior,TA)湿质量和肌纤维横截面积(muscle fiber cross-sectional area,FCSA),Western blot检测嘌呤霉素(puromycin,Puro)和泛素(ubiquitin,Ub)的含量,并对SOL和TA进行RNA测序,筛选差异基因,分析差异基因所富集的生物过程和通路。结果①干预期间,C组和H组体质量持续增长,但各时间点H组体质量始终低于C组,干预后H组瘦体质量及其百分比、抓力及相对抓力均低于C组,但差异无统计学意义。②H组TA湿质量较C组显著降低(P<0.05);H组SOL和TA肌纤维均出现损伤;SOL的FCSA显著低于C组(P<0.05)。③H组SOL中Puro和Ub均显著高于C组,Puro/Ub显著降低(P<0.05);TA中Puro/Ub显著增加(P<0.01)。④两组SOL中差异基因有665个,TA中差异基因有228个,在两种肌肉中均上调的差异基因有58个,均下调的有35个;H/C组SOL中上调差异基因功能主要富集于炎症反应和蛋白合成过程,参与调控的通路为TNF和Jak-STAT信号通路;H/C组TA中上调差异基因功能主要富集于抗氧化、糖代谢和糖皮质激素调控过程,参与调控的通路为PPAR信号通路和谷胱甘肽代谢过程,下调基因富集于快肌和慢肌之间的转化和氧化应激过程。结论慢性间歇性低氧可能通过增加炎症反应以促进比目鱼肌萎缩,而胫骨前肌可能通过提高抗氧化能力以抵抗低氧诱导的肌萎缩。ObjectiveTo compare the effects of chronic intermittent hypoxia exposure on different fiber types of muscle and investigate its possible mechanism.MethodsSixteen SD rats were randomly divided into normoxia control group(C group,placed in a normoxic chamber)and chronic intermittent hypoxia group(H group,placed in a hypoxic chamber with an oxygen concentration of 12.4%,8 h per day,for 4 weeks).Their body mass was weighed during the intervention,and the grasping force and lean body mass were tested after the intervention.The wet masses of soleus muscle(SOL)and musculi tibialis anterior(TA),muscle fiber cross-sectional area(FCSA)were measured.The protein levels of puromycin(Puro)and ubiquitin(Ub)were detected with Western blotting.RNA sequencing was carried out for TA tissues to screen the differential genes between the 2 groups.And the biological process and pathways enriched by these differential genes were analyzed.Results①During the intervention,the body mass of the C and H groups continued to increase,but it of the H group was always lower than that of the C group at each time point.The intervention also resulted in the decreases in the lean body mass and its percentage,grasping force and relative grasping force in the H group than the C group,but there were no statistical differences.②The wet mass of TA was significantly lower in the H group than the C group(P<0.05).In H group,both SOL and TA muscle fibers were damaged,and the FCSA of SOL was significantly lower than C group(P<0.05).③Puro and Ub in SOL tissues were obviously higher in the H group than the C group.The ratio of Puro/Ub was decreased significantly in SOL tissues(P<0.05),but increased significantly in TA tissues(P<0.01).④There were 665 differential genes found in SOL tissues and 228 differential genes in TA tissues between the 2 groups.Among them,58 differential genes were up-regulated and 35 were down-regulated in both muscles.Those up-regulated differential genes in SOL tissues were mainly enriched in inflammatory response and protein syn

关 键 词：慢性间歇性低氧暴露 骨骼肌 肌萎缩 慢肌纤维 快肌纤维 

分 类 号：R322.74[医药卫生—人体解剖和组织胚胎学] R363.22[医药卫生—基础医学]