不同雄株银杏叶提取液抗氧化能力及主要功能成分含量差异  

The differential analysis of antioxidant capacity and main functional components content of leaf extraction from different Ginkgo biloba male plants

作　　者：许继业 郁万文[1] 曹福亮[1,2] 汪贵斌 XU Jiye;YU Wanwen;CAO Fuliang;WANG Guibin(Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;The Jiangsu Province Platform for Construction and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037 [2]江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《食品与发酵工业》2022年第5期142-149,共8页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2017YFD0600701)。

摘　　要：以23个银杏雄株的叶片为材料,测定了不同单株间甲醇提取液的抗氧化能力及总黄酮、萜内酯、多糖、原花青素等功能性组分含量,分析了各单株间的抗氧化能力以及功能性组分含量的差异性,并通过聚类分析,筛选了抗氧化功能较好的优良单株。结果表明,银杏叶提取液的抗氧化能力及功能性成分含量在单株间均存在极显著差异(P<0.01),抗氧化活性的变异系数变幅为13.5%~37.04%、功能性组分含量的变异系数变幅为9.3%~15.63%,表现出明显的基因型差异;银杏叶提取液的DPPH自由基清除率、·O_(2)^(-)清除率、总还原力与总黄酮含量呈极显著中强度正相关(P<0.01),相关系数分别为0.52、0.70、0.62,说明总黄酮是银杏叶甲醇提取液抗氧化作用的主要物质基础;通过聚类分析将23个单株划分为4类,筛选出6#单株为抗氧化功能成分含量高且抗氧化活性强的类型。研究结果为高抗氧化银杏种质的评价与筛选提供了科学数据。In this study, 23 males gingkoes leaves were used as materials to study the antioxidant capacity and the contents of functional components(total flavonoids, terpene lactones, polysaccharides, proanthocyanidins) between Ginkgo biloba individuals’ methanol extract. Meanwhile, their differences in antioxidant capacity and the contents of functional components were analyzed. Finally, an excellent single plant with the better antioxidant function was screened out by cluster analysis. The results showed that the antioxidant capacity and functional component content of leaf extract were significantly different between individuals(P<0.01). Rich genetic diversity was found in G.biloba male plants, the variation coefficient of antioxidant capacity and functional components contents ranged from 13.5% to 37.04%, from 9.3% to 15.63%, respectively.Moreover, DPPH scavenging rate, ·O_(2)^(-)scavenging rate, total reducing the power of leaf extract were significantly positively correlated with total flavonoids content(P<0.01), the correlation coefficients were 0.52、0.70、0.62 respectively. The results indicated that total flavonoids were the main material basis of antioxidant capacity of G.biloba leaf extract. Furthermore, 23 G.biloba male plants were divided into 4 types by cluster analysis, and only 6# male plant showed high functional components contents and high antioxidant activity. These results provide a scientific basis for the screening of high antioxidant ginkgo germplasm and breeding high antioxidant ginkgo new varieties.

关 键 词：银杏雄株 叶提取液 抗氧化能力 功能性组分 

分 类 号：R284.1[医药卫生—中药学]