外源亚精胺对盐胁迫下茶树光合特性及关键酶基因表达的影响  

Effects of Exogenous Spermidine on Photosynthetic Characteristics and Gene Expression of Key Enzymes Under Salt Stress in Tea Plant

作　　者：杨妮 万绮雯 李逸民 韩妙华 滕瑞敏 刘洁霞 庄静 YANG Ni;WAN Qiwen;LI Yimin;HAN Miaohua;TENG Ruimin;LIU Jiexia;ZHUANG Jing(Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Tea Research Institute,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院,茶叶科学研究所,农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京210095 [2]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《园艺学报》2022年第2期378-394,共17页Acta Horticulturae Sinica

基　　金：江苏省农业科技自主创新资金项目[CX(20)3114];国家自然科学基金项目(31870681);江苏高校优势学科建设项目(PAPD)。

摘　　要：为了探讨外源亚精胺(Spd)对盐胁迫下茶树光合特性的调控机理,以茶树品种‘中茶108’为试验材料,通过Spd不同浓度(0、0.5、1.0、1.5 mmol·L^(-1))的喷施处理,研究盐胁迫(200 mmol·L^(-1)NaCl)下外源Spd对茶树的光合参数、叶绿素含量、气孔开度及关键酶基因表达的影响。结果显示:盐胁迫处理显著降低了茶树叶片的净光合速率(P_(n))、蒸腾速率(T_(r))及叶绿素含量等,提高了叶片的胞间CO_(2)浓度(C_(i)),处理初期(1、3 d)促进气孔开度,后期(5、9 d)逐渐抑制气孔开度;盐胁迫下,外源Spd显著提高了茶树的P_(n)、T_(r)、气孔导度(G_(s))、叶绿素a、b及总含量;外源Spd可以不同程度地诱导CsRbcL、CsTK、CsFBPase、CsPRK等光合碳同化基因的表达,并且缓解了盐胁迫对大多叶绿素合成基因抑制的影响。研究表明叶面喷施外源Spd可以有效缓解叶绿素的降解,促进茶树的光合作用,提高茶树的耐盐性,其中以喷施0.5 mmol·L^(-1)亚精胺的缓解效应最为显著。In order to explore the regulatory effects of exogenous spermidine(Spd)on the photosynthetic characteristics under salt stress of tea plant,tea variety‘Zhongcha 108’was used as the experimental material in this study. Through different concentrations(0,0.5,1.0,1.5 mmol · L^(-1))Spd spray treatments,the effects of exogenous Spd on tea photosynthetic parameters,chlorophyll content,stomatal aperture and the expression levels of key enzyme genes under salt stress(200 mmol · L^(-1) NaCl)were detected and analyzed. The results showed that the net photosynthetic rate(P_(n)),transpiration rate(Tr),and chlorophyll content were significantly reduced,and the intercellular CO_(2) concentration(C_(i))was increased under salt stress. Stomatal aperture was promoted the initial treatment(1,3 d),and inhibited gradually in the later period(5,9 d). After spraying exogenous Spd,P_(n),T_(r),stomatal conductance(G_(s)),chlorophyll a,chlorophyll b and Chlorophyll(a + b)contents were significantly increased under salt stress.Exogenous Spd can induce the up-regulation of CsRbcL, CsTK, CsFBPase, CsPRK and other photosynthetic carbon assimilation genes to varying degrees,and alleviate the influence of salt stress on the inhibition most chlorophyll synthesis genes. The study demonstrated that spraying exogenous Spd can effectively alleviate the degradation of chlorophyll,promote photosynthesis,and improve the salt tolerance of tea plant,of which 0.5 mmol · L^(-1) Spd was the most effective.

关 键 词：茶树 亚精胺 盐胁迫 气孔开度 光合特性 

分 类 号：S571.1[农业科学—茶叶生产加工]