绵羊Myf5基因多态性与胴体性状的相关性分析  

Correlation analysis of sheep Myf5 gene polymorphism and carcass traits

作　　者：任思睿 任亭亭 胡慧宇 刘明军 李文蓉 张宁 张雪梅 刘晨曦 贺三刚 REN Sirui;REN Tingling;HU Huiyu;LIU Mingjun;LI Wenrong;ZHANG Ning;ZHANG Xuemei;LIU Chenxi;HE Sangang(Institute of Biotechnology/Key I^ihoratory of Genetic Breeding and Reproduction of Herbivorous Livestock,Ministry of Agriculture and Rural Affairs/Xinjiang Key Laboratory of Animal Biotechnology,Xinjiang Academy of Animal Science,Urumqi 830011,China;College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆畜牧科学院生物技术研究所/农业农村部草食家畜遗传育种与繁殖重点实验室/新疆动物生物技术重点实验室,乌鲁木齐830011 [2]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《黑龙江畜牧兽医》2022年第2期38-42,135,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：政府间国际科技创新合作重点专项(2017YFE0117900);新疆维吾尔自治区天山创新团队计划项目(2018D14004)。

摘　　要：为研究特克赛尔×哈萨克横交F2代绵羊Myf5基因多态性(single nucleotide polymorphisms, SNPs)及其与胴体性状之间的关系,试验采用PCR测序技术和飞行时间质谱检测分型方法对434只特克赛尔×哈萨克横交F2代绵羊的Myf5基因SNPs进行分型,并将分型结果与其胴体性状(胴体重、胴体长、踝骨宽、臀宽、胸宽、肩宽和胸深)进行关联分析。结果表明:在Myf5基因上共发现了5个SNPs位点,即A116460176G、rs604841316、rs416158998、rs588855095和rs412277497。其中rs416158998属于内含子突变,在特克赛尔×哈萨克横交F2代绵羊中表现为中度多态[0.25<多态信息含量(PIC)<0.5],不处于Hard-Weinberg平衡状态(P≤0.05),与胸宽、肩宽和臀宽呈显著或者极显著相关(P<0.05或P<0.01)。rs588855095属于内含子突变,在绵羊中表现为低度多态(PIC≤0.25),处于Hard-Weinberg平衡状态(P>0.05),与臀宽呈显著相关(P<0.05)。rs412277497属于外显子上丝氨酸到半胱氨酸的错义突变,在绵羊中表现为中度多态(0.250.05),与胴体长、胸宽和肩宽呈显著或极显著相关(P<0.05或P<0.01)。A116460176G和rs604841316与胴体性状相关性不显著(P>0.05)。说明Myf5基因中rs416158998、rs588855095和rs412277497位点可以为培育优良新品种绵羊提供可参考的遗传标记。In order to study the rlationship between singe nucleotide polymorphisms(SNP's)of My(5 gene and careas tnits of Texelx Kazakh Cross F2 generation sheep,PCR sequencing technology and time-of-flight mass spectrometry dection method were used to type Myf5 gene SNPs in 434 Texelx Kazakh cross F2 generation sheeps,and correlation analysis was conducted between the easifieation resuls and is careass trails(carcass weight,careass length,ankle width,hip width,chest width,shoulder width and chest depth).Resuls showed that a total of five.SNPs loci were found in the Myf5 gene,namely Al 16460176C,r>604841316.rs416158998.r588855095 and rs412277497.Among them,r>416158998,an intron mutation,was moderately polymorphice in Texelx Kazakh cross F2 generation sheep(0.25<PIC<0.5).not in a Hard-W einberg equilibrium state(P≤0.05),and signifcantly or extremelly signifcanly orrelated with chest width,shoulder width and hip width(P<0.05 or P<0.01).r588855095,an intron mutation,was low polymophism in sheep(PIC≤0.25),in a Hard-Weinberg equilibrium state(P>0.05),and signifcantly correlated with hip widh(P<0.05).r412277497.a missense mutation with serine mutated to cystein on the exon,was moderately polymorphie in sheep(0.25<PIC<0.5).in a Hard-W einberg equilibriumn state(P>0.05).and significantly or ex-tremelly signifcantly crrelated with carcass lengh,chest width and shoulder widh(P<0.05 or P<0.01).A1164601766和rs604841316 was not signifieantly correlated with earcass trais(P>0.05).The experiment revealed that n416158998,r588855095 and rs412277497 can provide reference genetic markers for breeding new breeds of sheep.

关 键 词：特克赛尔×哈萨克横交F2代绵羊 Myf5基因 核苷酸多态性 胴体性状 相关性分析 

分 类 号：S826[农业科学—畜牧学]