3种花色类型滇黄精的遗传关系研究  

Genetic Relationship of Polygonatum kingianum with Three Different Flower Colors

作　　者：谢蕾 肖良俊 吴涛 李贤忠[1] 苏包顺 刘志彤 XIE Lei;XIAO Liang-jun;WU Tao;LI Xian-zhong;SU Bao-shun;LIU Zhi-tong(Southwest Forestry University,Kunming 650224,China;Yunnan Academy of Forestry and Grassland,Kunming 650201,China;Dali Linyun Biotechnological Development Company Ltd of Yunnan,Dali 671000,China;Weishan Agricultural and Rural Affaires Bureau of Yunnan,Dali,671000,China)

机构地区：[1]西南林业大学,云南昆明650224 [2]云南省林业和草原科学院,云南昆明650201 [3]大理市林韵生物科技开发有限责任公司,云南大理671000 [4]巍山彝族回族自治县农业农村局园艺工作站,云南大理671000

出　　处：《浙江林业科技》2022年第2期21-26,共6页Journal of Zhejiang Forestry Science and Technology

基　　金：云南省基础研究计划面上项目(202001AT070146)。

摘　　要：滇黄精Polygonatum kingianum是我国传统名贵中药材,也是最具云南特色的药用植物资源。在大理州大理市挖色镇进行滇黄精资源收集和扩繁过程中,发现滇黄精资源在花被颜色上存在紫红花、白花和白花带紫斑3种类型,其中花被白花带紫斑为新发现类型。为明确3种花色滇黄精的遗传关系,对3种花色滇黄精的48个植株进行ISSR分子标记分析。结果表明,11条ISSR引物对48个植株样品进行扩增,共获得103条清晰条带,其中多态性条带有99条,多态性比率为96.1%,遗传一致度为0.89~0.91。遗传差异和UPGMA聚类分析显示,白花类型(P1)、白花带紫斑类型(P2)与紫红花类型(P3)的滇黄精均各自独立聚为一组,遗传距离上白花带紫斑类型(P2)与紫红花类型(P3)的亲缘关系较近,可知白花带紫斑类型(P2)是紫红花类型(P3)和白花类型(P1)的过渡类型或是白花类型与紫红花类型自然授粉所产生子代的一种杂合状态。本研究结果为滇黄精资源的遗传关系研究及分类鉴别奠定了一定基础。In July 2019,Polygonatum kingianum with flower of purple safflower,white flowers and purple spots on white flowers were collected 48 individuals each in Dali,Yunnan province for ISSR molecular marker analysis.The results showed that a total of 103 bands were obtained from 48 P.kingianum individuals amplified with 11 ISSR primers,among which,99 bands were polymorphic,and the genetic identity was 0.89-0.91.The genetic difference and UPGMA clustering analysis showed that the white flower genotype(P1),the white flower with purple spot genotype(P2)and the purple safflower genotype(P3)could be grouped into one group,among which,there was closer genetic relationship between P2 and P3.Therefore,the P2 might be the transitional genotype between P3 and P1,or a heterozygous state of the offspring produced by the plants of the white flower genotypes after receiving the pollen of the purple safflower.

关 键 词：滇黄精 花色类型 ISSR分子标记 遗传关系 UPGMA聚类分析 

分 类 号：S759.82[农业科学—森林经理学]