SOX7在肾细胞癌中的表达及其对肾癌细胞生物学行为的影响  

Expression of SOX7 in renal cell carcinoma and its effect on the biological behavior of renal cancer cells

作　　者：张晓宇[1] 苏建志[1] 任宗涛[1] 刘彬[1] 张爱莉[1] ZHANG Xiaoyu;SU Jianzhi;REN Zongtao;LIU Bin;ZHANG Aili(Department of Urology, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050000, China)

机构地区：[1]河北医科大学第四医院泌尿外科,河北石家庄050000

出　　处：《江苏大学学报（医学版）》2022年第2期124-130,135,共8页Journal of Jiangsu University：Medicine Edition

基　　金：河北省卫生厅科技成果推广课题(20201100)。

摘　　要：目的:研究Y性别决定基因7(sex determining region Y-box 7,SOX7)在肾细胞癌的表达以及对肾癌细胞生物学行为的影响。方法:qRT-PCR和甲基化特异性PCR(MSP)检测SOX7在肾细胞癌组织和肾癌细胞(A498、ACHN、786-O)中的mRNA表达及甲基化状态;免疫组织化学法(immunohistochemistry,IHC)检测SOX7在肾细胞癌组织中的蛋白表达。采用qRT-PCR方法检测5-Aza-dC/TSA干预后肾癌细胞的SOX7 mRNA表达。在肾癌细胞ACHN中转染SOX7过表达质粒后,采用MTS、克隆形成、划痕实验和Transwell实验检测ACHN细胞增殖、迁移和侵袭的变化。结果:qRT-PCR及IHC结果显示,SOX7在肾癌组织中mRNA和蛋白的表达显著低于癌旁组织(P<0.01),并与淋巴结转移和TNM分期相关(P<0.05)。SOX7 mRNA在A498、ACHN、786-O细胞中的表达量均明显低于对照组HK-2细胞(P<0.01)。5-Aza-dC/TSA处理后肾癌细胞SOX7 mRNA表达均增高(P<0.01)。MSP结果显示,SOX7在肾癌组织中的甲基化率明显高于癌旁组织(P<0.01),并与TNM分期相关(P<0.05)。过表达SOX7可抑制ACHN细胞的增殖活性、迁移能力和侵袭能力。结论:SOX7在肾细胞癌中低表达,SOX7的高甲基化可能是肾癌发生的分子机制之一。Objective:To investigate the expression of sex determining region Y-box 7(SOX7)in renal cell carcinoma and the effect on the biological behavior of renal cancer cells.Methods:qRT-PCR and methylation-specific PCR(MSP)were used to detect the expression and methylation status of SOX7 in renal cell carcinoma tissues and renal cancer cells(A498,ACHN,786-O);immunohistochemistry(IHC)was used to detect the protein expression of SOX7 in renal cell carcinoma tissues.The SOX7 mRNA expression in renal cancer cells after 5-Aza-dC/TSA intervention was also detected by qRT-PCR.After transfection of SOX7 overexpression plasmid in renal cancer cells ACHN,cell proliferation,migration and invasion were detected by MTS,clone formation,scratch assay and Transwell assay,respectively.Results:The qRT-PCR and IHC results showed that the expression of SOX7 mRNA and protein in renal cancer tissues was significantly lower than that in paraneoplastic tissues(P<0.01)and correlated with lymph node metastasis and TNM grade(P<0.05).SOX7 mRNA expression in three renal cancer cells(A498,ACHN,786-O)was significantly lower than that in the control group HK-2(P<0.01).SOX7 mRNA expression was increased after 5-Aza-dC/TSA treatment in all(P<0.01).MSP results showed that the methylation rate of SOX7 was significantly higher in kidney cancer tissues than that in paraneoplastic tissues(P<0.01)and correlated with TNM stage(P<0.05).Overexpression of SOX7 inhibited the proliferative activity,migration ability and invasive ability of ACHN.Conclusion:SOX7 is lowly expressed in renal cell carcinoma,hypermethylation of SOX7 may be one of the molecular mechanisms of renal carcinoma histogenesis.

关 键 词：SOX7 肾细胞癌 免疫组化 甲基化状态 功能实验 

分 类 号：R737.11[医药卫生—肿瘤]