miR-182对前列腺癌PC-3细胞增殖凋亡迁移的影响及机制研究  

Effect of MiR-182 on the Proliferation Apoptosis and Migration of Prostate Cancer PC-3 Cells and Its Mechanism

作　　者：甘卓 李艳平[1] 史冰[1] 王群[1] GAN Zhuo;LI Yanping;SHI Bing(The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Hunan Changsha 410007, China)

机构地区：[1]湖南中医药大学第一附属医院核医学科,湖南长沙410007

出　　处：《河北医学》2022年第3期353-357,共5页Hebei Medicine

基　　金：湖南省卫生健康委科研立项课题,(编号:20200469)。

摘　　要：目的:探讨微小RNA-182(miR-182)对前列腺癌PC-3细胞增殖、凋亡、迁移的影响及可能的作用机制。方法:将对数生长期的前列腺癌PC-3细胞随机分为:对照组、miR-182低表达载体(miR-182 inhibitor)组、miR-182过表达载体(miR-182 mimics)组,各组进行相应处理后,培养72h;测定细胞存活率、单克隆形成数目、凋亡率、迁移能力、miR-182、异黏蛋白(MTDH)mRNA和蛋白表达水平。结果:与对照组比较,miR-182 inhibitor组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显降低(P<0.05),细胞凋亡率明显升高(P<0.05);miR-182 mimics组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显升高(P<0.05),细胞凋亡率明显降低(P<0.05)。与miR-182 inhibitor组比较,miR-182 mimics组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显升高(P<0.05),细胞凋亡率明显降低(P<0.05)。结论:下调miR-182表达可抑制前列腺癌PC-3细胞增殖、迁移,诱导其凋亡;其机制可能与抑制MTDH mRNA和蛋白的表达有关;上调miR-182表达可逆转前列腺癌PC-3细胞的生物学行为。Objective:To investigate the effect of microRNA-182(miR-182)on the proliferation,apoptosis and migration of prostate cancer PC-3 cells and its possible mechanism.Methods:Prostate cancer PC-3 cells in logarithmic growth stage were randomly divided into control group,miR-182 low expression vector(miR-182 inhibitor)group and miR-182 overexpression vector(miR-182 mimics)group.After corresponding treatment,each group was cultured for 72 hours;the cell survival rate,monoclonal forming number,apoptosis rate,migration ability,miR-182,metadherin(MTDH)mRNA and protein expression level were measured.Results:Compared with the control group,the cell survival rate,monoclonal forming number,number of membrane penetration,the expression levels of miR-182,MTDH mRNA and protein in miR-182 inhibitor group were significantly lower(P<0.05),and the apoptosis rate was significantly higher(P<0.05);the cell survival rate,monoclonal forming number,number of membrane penetration,the expression levels of miR-182,MTDH mRNA and protein in miR-182 mimics group were significantly higher(P<0.05),and the apoptosis rate was significantly lower(P<0.05).Compared with miR-182 inhibitor group,the cell survival rate,monoclonal forming number,number of membrane penetration,the expression levels of miR-182,MTDH mRNA and protein in miR-182 mimics group were significantly higher(P<0.05),and the apoptosis rate was significantly lower(P<0.05).Conclusion:Down regulation of miR-182 expression can inhibit the proliferation,migration and induce apoptosis of prostate cancer PC-3 cells;the mechanism may be related to the inhibition of MTDH mRNA and protein expression;up regulation of miR-182 expression can reverse the biological behavior of prostate cancer PC-3 cells.

关 键 词：微小RNA-182 前列腺癌PC-3细胞 增殖 凋亡 迁移 异黏蛋白 

分 类 号：R73[医药卫生—肿瘤]