β-隐黄素对大鼠正畸牙移动模型RANKL RANK OPG通路及破骨细胞形成的影响  

Effects ofβ-Cryptoxanthin on RANKL/RANK/OPG Pathway and Osteoclast Formation in Orthodontic Tooth Movement Model of Rats

作　　者：王帅 常颖[1] 席光伟[1] 沈娜[1] WANG Shuai;CHANG Ying;XI Guangwei(Aerospace Center Hospital, Beijing 100049, China)

机构地区：[1]航天中心医院口腔科,北京100049

出　　处：《河北医学》2022年第3期362-367,共6页Hebei Medicine

基　　金：北京市科学技术委员会科研计划项目,(编号:D191100000413007)。

摘　　要：目的:探究β-隐黄素对大鼠正畸牙移动模型核因子κB受体活化因子配体(RANKL)、核因子κB受体活化因子(RANK)、骨保护素(OPG)及对破骨细胞形成的影响。方法:将大鼠按随机数表法分为:正常组、模型组、β-隐黄素低(3μg)、中(6μg)、高(12μg)剂量组,每组10只。除正常组外,其余各组建立大鼠正畸牙移动模型,安装矫治器后第1天开始给药,β-隐黄素低、中、高剂量组分别于大鼠左侧上颌第一磨牙近中牙龈黏膜下注射3μg、6μg、12μg的β-隐黄素;正常组和模型组注射等量生理盐水;每2天给药1次,连续给药28d。各组大鼠于末次给药24h后,检测大鼠正畸牙移动距离;取大鼠双侧上颌第一磨牙牙根处牙周膜及牙槽骨组织,行苏木精-伊红(HE)染色和抗酒石酸酸性磷酸酶(TRAP)染色,光镜下计数压力侧及张力侧牙槽骨表面破骨细胞数目;蛋白免疫印迹法检测牙根处牙周膜及牙槽骨组织RANKL、RANK、OPG蛋白表达水平。结果:与正常组相比,模型组大鼠压力侧牙周间隙变窄,张力侧牙周韧带增宽,可见成骨反应,压力侧及张力侧TRAP染色呈强阳性,且压力侧TRAP染色较张力侧深,正畸牙移动距离、压力侧及张力侧牙槽骨表面破骨细胞数目、牙根处牙周膜及牙槽骨组织RANKL、RANK、OPG蛋白表达水平及RANKL/OPG比值均升高(P<0.05)。与模型组相比,β-隐黄素低、中、高剂量组大鼠张力侧新骨形成逐渐明显,压力侧TRAP染色呈强阳性,张力侧TRAP染色呈弱阳性,正畸牙移动距离、牙根处牙周膜及牙槽骨组织RANKL、OPG蛋白表达水平升高(P<0.05),张力侧牙槽骨表面破骨细胞数目、RANKL/OPG比值降低(P<0.05);且β-隐黄素各剂量组呈剂量依赖性变化(P<0.05)。结论:β-隐黄素可能通过上调RANKL及OPG表达,降低RANKL/OPG比值,阻断RANKL与RANK结合,促进矫正牙移动,抑制破骨细胞进一步活化,来维持牙周膜重塑和牙槽骨重建中成骨形成与破骨吸收Objective:To investigate the effects ofβ-cryptoxanthin on receptor activator of NF-κB ligand(RANKL)/receptor activator of NF-κB(RANK)/osteoprotegerin(OPG)and osteoclast formation in orthodontic tooth movement model of rats.Methods:The rats were divided by random number table method into normal group,model group,β-cryptoxanthin low(3μg),medium(6μg)and high(12μg)dose groups,with 10 rats in each group.Except for the normal group,all the other groups were established as orthodontic tooth movement models,and the administration ofβ-cryptocanthin was started on the first day after the installation of orthodontic appliances.3μg,6μg and 12μg ofβ-cryptocanthin were injected under the gingival mucosa of the left maxillary first molar near the middle of the rats in the low,medium and high dose groups respectively;the normal and model groups were injected with equal amounts of saline;the drug was administered once every 2 days for 28 days.The orthodontic tooth movement distance was measured 24h after the last dose in each group of rats.Periodontal membrane and alveolar bone tissues at the roots of maxillary first molars were taken from both sides and stained with hematoxylin-eosin(HE)and anti-tartrate acid phosphatase(TRAP),and the number of osteoclasts on the surface of alveolar bone on the pressure and tension sides were counted under light microscope.The expression levels of RANKL,RANK and OPG proteins in periodontal ligament and alveolar bone tissue at root were detected by Western blot.Results:Compared with the normal group,the periodontal space was narrowed on the pressure side and the periodontal ligament was widened on the tension side,osteogenic reactions were seen,TRAP staining was strongly positive on the pressure and tension sides,and TRAP staining was deeper on the pressure side than on the tension side.The levels of RANKL,RANK,OPG and RANKL/OPG ratio were all increased(P<0.05).Compared with the model group,the formation of new bone on the tension side of rats in theβ-cryptoxanthin low,medium and hig

关 键 词：β-隐黄素 正畸牙移动 大鼠 核因子ΚB受体活化因子配体 核因子ΚB受体活化因子 骨保护素 破骨细胞 

分 类 号：R78[医药卫生—口腔医学]