Meyerozyma sp.合成Ag_(2)S量子点及其抗菌性  

Synthesis and antibacterial activity of Ag_(2)S quantum dots by Meyerozyma sp.

作　　者：张小雪 孙维良 张友德 武超[1,2] 邓国志 ZHANG Xiaoxue;SUN Weiliang;ZHANG Youde;WU Chao;DENG Guozhi(School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China;Anhui Provincial Key Laboratory of Wetland Ecological Protection and Restoration (Anhui University),Hefei 230601, China;Anhui Xinyu Ecological Industry Co., Ltd., Hefei 230601, China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]安徽大学湿地生态保护与修复安徽省重点实验室,合肥230601 [3]安徽新宇生态产业股份有限公司,合肥230601

出　　处：《功能材料》2022年第2期2187-2192,共6页Journal of Functional Materials

基　　金：国家自然科学基金项目(31670126);湿地生态保护与修复安徽省重点实验室开放基金(AKLWEPR-K-2020-02);安徽省住房城乡建设科学技术计划项目(2017YF-03)共同资助。

摘　　要：利用从大庆油田土壤中筛出的耐重金属真菌季也蒙毕赤酵母(Meyerozyma sp.)还原硝酸银和亚硫酸钠合成硫化银(Ag_(2)S)量子点,并通过X射线光电子能谱分析(XPS)、X射线衍射(XRD)、倒置荧光显微镜、扫描电镜(SEM)对合成的硫化银(Ag_(2)S)量子点进行表征,同时研究了合成的硫化银(Ag_(2)S)量子点的抗菌性。结果显示,通过Meyerozyma sp.菌合成Ag_(2)S QDs,具有单斜α-Ag_(2)S晶型,在410 nm左右处具有最大吸收峰,对铜绿假单胞菌、大肠杆菌分别在30和90 min时抗菌率达到100%,对芽孢杆菌在2 h内抗菌率约为99.9%。通过SEM分析硫化银(Ag_(2)S)量子点使细菌细胞表面发生凹陷、破碎。研究表明硫化银(Ag_(2)S)量子点对革兰阴性菌包括Escherichia coli和Pseudomonas aeruginosa以及革兰氏阳性菌Bacillus均有显著的抗菌性,可能是由于小粒径的硫化银(Ag_(2)S)量子点吸附在细菌细胞膜表面,造成膜的损伤,进而导致细胞死亡。Silver based nanomaterials have excellent bactericidal potential and can be used as antibacterial agents.Therefore,it is of great significance to synthesize silver sulfide nanomaterials by a simple,environmentally friendly and economical method.In this paper,silver sulfide quantum dots are biosynthesized using silver nitrate and sodium sulfite as precursors,and their possible antibacterial properties are revealed.Meyerozyma sp.,a heavy metal tolerant fungus screened from soil of Daqing Oilfield,is used.Silver sulfide(Ag_(2)S)quantum dots are biosynthesized by reducing silver nitrate and sodium sulfite.The synthesized silver sulfide(Ag_(2)S)quantum dots are characterized by UV-vis,X-ray photoelectron spectroscopy(XPS),X-ray diffraction(XRD),inverted fluorescence microscope and scanning electron microscope(SEM).The antibacterial activity of silver sulfide(Ag_(2)S)quantum dots is studied by disk diffusion method and drop plate sterilization.The results show that the silver sulfide(Ag_(2)S)quantum dots synthesized by Meyerozyma sp.are monoclinicα-Ag_(2)S crystals with the highest absorption peak at 410 nm.With the increase of the concentration of silver sulfide quantum,the diameter of the inhibition zone increases,and the antibacterial activity against gram-negative bacteria and gram-positive bacteria increases.The inhibition rate of silver sulfide(Ag_(2)S)quantum dots against Pseudomonas aeruginosa and Escherichia coli reaches 100%at 30 min and 90 min respectively.The inhibition rate of bacillus was 99.9%within 2 h.The possible antibacterial mechanism of silver sulfide(Ag_(2)S)quantum dots against bacteria is analyzed by SEM.The results show that silver sulfide(Ag_(2)S)quantum dots inhibit and damage the cell surface of Gram-negative bacteria such as Escherichia coli,Pseudomonas aeruginosa and Gram-positive bacillus,and show significant antibacterial activity,which may be due to the small particle size of silver sulfide(Ag_(2)S)quantum dots.It attaches to the surface of the cell membrane of bacteria,causing damage

关 键 词：生物合成 Ag_(2)S QDs 季也蒙 细菌 抗菌性 

分 类 号：TB383[一般工业技术—材料科学与工程] X172[环境科学与工程—环境科学]