盘基网柄菌RasD蛋白的生物信息学分析及趋电性研究  

Bioinformatics Analysis and Electrotaxis Research of RasD Protein of Dictyostelium discoideum

作　　者：王家家 葛晓雪 王晓燕[1] 高晶 高润池[1] WANG Jia-jia;GE Xiao-xue;WANG Xiao-yan;GAO Jing;GAO Run-chi(School of Life Science,Yunnan Normal University,Kunming 650500,China;School of Agriculture,Yunnan University,Kunming 650500,China)

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]云南大学农学院,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2022年第2期66-72,共7页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(31601130,31960144).

摘　　要：通过对盘基网柄菌(Dictyostelium discoideum)RasD蛋白进行生物信息学分析及趋电性研究,探究盘基网柄菌能否作为开发电激治疗肿瘤的生物模型.研究发现RasD蛋白含有187个氨基酸残基,是亲水性蛋白,二级结构主要以α螺旋和无规则卷曲为主,同源比较发现盘基网柄菌细胞中的RasD蛋白与人类的Ras蛋白相似性高达72%,在12 V/cm的电场中rasD缺陷型细胞株定向迁移的速度增加,方向性降低.结果表明可以用盘基网柄菌来研究肿瘤内源性生物电场指导细胞定向迁移的机制.This study analyzed the RasD protein of Dictyostelium discoideum using bioinformatics and electrotaxis research to determine whether Dictyostelium discoideum is a suitable model for the development of electric stimulation cancer treatment.The results showed RasD to be a hydrophilic protein containing 187 amino acid residues.Its secondary structure was mainly alpha helix and random coils.Sequence alignment using BLAST showed that RasD protein in the cells of Dictyostelium discoideum was 72%identical to Ras protein in human.An examination of gene regulation of the electrotaxis of Dictyostelium discoideum cells showed a reduced response to directedness and an increased response to track speed in rasD null cell in an electric field of 12 V/cm.These results indicated that Dictyostelium discoideum could be used to study the mechanism by which endogenous bioelectric fields in tumors guide the directional migration of cells.

关 键 词：盘基网柄菌 肿瘤生物电 趋电性 RasD RAS 

分 类 号：Q936[生物学—微生物学] Q811.4