Application of acidic protease in the pickling to simplify the pelt bating process  

作　　者：Xu Zhang Mengchu Gao Sadaqat Ali Chattha Yiwen Zhu Biyu Peng Yongbin Ye 

机构地区：[1]National Engineering Research Center of Clean Technology in Leather Indus-try,Sichuan University,Chengdu 610065,Sichuan,People’s Republic of China [2]Key Laboratory of Leather Chemistry and Engineering of Ministry of Educa-tion,Sichuan University,Chengdu 610065,Sichuan,People’s Republic of China [3]Zhejiang Tongtianxing Group Joint-Stock Co.,Ltd.,Quzhou 324022,Zhejiang,People’s Republic of China

出　　处：《Journal of Leather Science and Engineering》2021年第1期298-311,共14页皮革科学与工程（英文）

基　　金：This work was financially supported by the National Key Research and Devel-opment Program of China(2017YFB0308402).

摘　　要：Traditionally,universally used pelt bating technologies rely on the application of trypsin,neutral and alkaline microbial proteases but suffer from complicated operation,limited bating efficiency and unsatisfac-tory leather performance.Therefore,devising a new pelt bating approach to achieve high bating efficiency and excellent leather performance has always been wished for by the leather industry.To pursue this goal,years of persistent research work enabled us to develop a novel approach for pelt bating by means of acidic proteases in pickling process.Initially,basic enzymatic characteristics and bating effectiveness of several typical acidic pro-teases in pelt pickling medium were investigated;then,the bating effectiveness through the quantitative charac-terization of protease activity of the optimal acidic protease was compared with that of the conventional bating enzyme.The results indicated that all of the selected acidic proteases had good salt-tolerance and exhibited optimum activity at pH 3.0–4.0.The novel pickling-bating method based on microbial origin acidic protease L80A led to an outstanding performance on pelt bating at the dosage of 150 U/mL of collagenolytic activity.The bating effectiveness of acidic protease L80A was comparable to and even better than that of trypsin BEM due to its mod-erate proteolytic ability.Moreover,the deep and even penetration of acidic protease in the pelt permitted it to produce soft,organoleptically stable and overall better quality crust leather than that of the conventional trypsin bating method.Additionally,pelt bating was performed along with the pickling process without extra inactiva-tion and washing operation,making the bating operation more efficient,economical,and environment friendly.Results had made us to conclude that this cutting-edge acidic proteases based pickling-bating method could be the first step/way forward to replace the decades-old traditional pelt bating technology.

关 键 词：Bating TRYPSIN PICKLING Acidic protease Collagenolytic activity 

分 类 号：S86[农业科学—野生动物驯养]