Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows  

作　　者：Lorena Padilla Marina López-Arjona Silvia Martinez-Subiela Heriberto Rodriguez-Martinez Jordi Roca Isabel Barranco 

机构地区：[1]Department of Medicine and Animal Surgery,Faculty of Veterinary Medicine,University of Murcia,E-30100 Murcia,Spain [2]Department of Biomedical&Clinical Sciences(BKV),BKH/Obstetrics&Gynaecology,Faculty of Medicine and Health Sciences,Linköping University,SE-58185 Linköping,Sweden [3]Department of Veterinary Medical Sciences,University of Bologna,IT-40064 Ozzano dell’Emilia,Bologna,Italy

出　　处：《Journal of Animal Science and Biotechnology》2022年第2期430-440,共11页畜牧与生物技术杂志（英文版）

基　　金：supported by MICINN and FEDER [AGL2016-79096-R and PID2020-113493RB-I00] Madrid (Spain);Seneca Foundation [19892/GERM/15]Murcia (Spain);European Union’s Horizon 2020 research and innovation program (H2020-MSCA-IF-2019-891382);financially supported by MINECO (BES-2016-076404);MECD (FPU16/02170),Spain,respectively。

摘　　要：Background:Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry.Concentrations of the small peptide hormone oxytocin(OXT),involved in male reproductive function and present in the seminal plasma(SP)of several species could be a robust one.This study characterized concentrations of SPOXT in ejaculates from boars used in artificial insemination(AI)programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored AI-semen.Seminal OXT concentrations(ng/mL)were measured in 169 ejaculates from 61 boars of the Duroc,Pietrain,Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA®technology.Ejaculate(ejaculate volume,sperm concentration,total sperm count)and sperm parameters(motility,viability,intracellular generation of reactive oxygen species,plasma membrane fluidity)were assessed at 0 h and 72 h in AI-semen samples stored at 17℃.In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated>100 sows and evaluated both farrowing rate and litter size of 3,167 sows.Results:The results showed that SP-OXT differed between boars and between ejaculates within boar(P<0.05)but not between breeds(Duroc,Pietrain,Landrace and Large White).Ejaculates with higher SP-OXT concentration/mL(hierarchically grouped;P<0.001)had larger volume and came from younger boars(P<0.05).Ejaculates of boars showing positive farrowing rate deviation exhibited higher(P<0.05)SP-OXT concentration/mL than those with negative farrowing rate deviation.Conclusion:The SP concentrations of OXT are boar,ejaculate and age dependent,and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

关 键 词：Artificial insemination EJACULATE FERTILITY OXYTOCIN PIG Semen quality Seminal plasma 

分 类 号：S828.34[农业科学—畜牧学]