25-hydroxycholecalciferol reverses heat induced alterations in bone quality in finisher broilers associated with effects on intestinal integrity and inflammation  

作　　者：Huaiyong Zhang Maryam Majdeddin Djoere Gaublomme Bernard Taminiau Matthieu Boone Dirk Elewaut George Daube Iván Josipovic Keying Zhang Joris Michiels 

机构地区：[1]Laboratory for Animal Nutrition and Animal Product Quality,Department of Animal Sciences and Aquatic Ecology,Ghent University,9000 Ghent,Belgium [2]Key laboratory of Animal Disease-resistant Nutrition,Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Ya’an 611130,Sichuan,China [3]Unit Molecular Immunology and Inflammation,VIB Center for Inflammation Research,Ghent University and Department of Rheumatology,Ghent University Hospital,9000 Ghent,Belgium [4]Department of Food Sciences-Microbiology,University of Liège,4000 Liège,Belgium [5]Ghent University Centre for X-ray Tomography(UGCT),Ghent University,9000 Ghent,Belgium [6]Department of Physics and Astronomy,Radiation Physics Research Group,Ghent University,9000 Ghent,Belgium

出　　处：《Journal of Animal Science and Biotechnology》2022年第2期499-519,共21页畜牧与生物技术杂志（英文版）

基　　金：This work was supported by the China Scholarship Council(CSC,[2019]110)The support by DSM Nutritional Products(Basel,Switzerland)for the analysis of vitamin D3 and metabolites is well appreciated;The Ghent University Special Research Fund is acknowledged for the financial support to the UGCT Centre of Expertise(BOF.EXP.2017.0007).

摘　　要：Background:Alterations in ambient temperature have been associated with multiple detrimental effects on broilers such as intestinal barrier disruption and dysbiosis resulting in systemic inflammation.Inflammation and 25-hydroxycholecalciferol(25-OH-D_(3))have shown to play a negative and positive role,respectively,in the regulation of bone mass.Hence the potential of 25-OH-D_(3)in alleviating heat induced bone alterations and its mechanisms was studied.Results:Heat stress(HS)directly induced a decrease in tibia material properties and bone mass,as demonstrated by lower mineral content,and HS caused a notable increase in intestinal permeability.Treatment with dietary 25-OH-D_(3)reversed the HS-induced bone loss and barrier leak.Broilers suffering from HS exhibited dysbiosis and increased expression of inflammatory cytokines in the ileum and bone marrow,as well as increased osteoclast number and activity.The changes were prevented by dietary 25-OH-D_(3)administration.Specifically,dietary 25-OH-D_(3)addition decreased abundance of B-and T-cells in blood,and the expression of inflammatory cytokines,especially TNF-α,in both the ileum and bone marrow,but did not alter the diversity and population or composition of major bacterial phyla.With regard to bone remodeling,dietary 25-OH-D_(3)supplementation was linked to a decrease in serum C-terminal cross-linked telopeptide of type I collagen reflecting bone resorption and a concomitant decrement in osteoclast-specific marker genes expression(e.g.cathepsin K),whereas it did not apparently change serum bone formation markers during HS.Conclusions:These data underscore the damage of HS to intestinal integrity and bone health,as well as that dietary 25-OH-D_(3)supplementation was identified as a potential therapy for preventing these adverse effects.

关 键 词：Bone remodeling HS INFLAMMATION Intestinal barrier Tibial mass 

分 类 号：S831[农业科学—畜牧学]