双重PCR检测海产品中携带trh毒力基因副溶血性弧菌的研究  

Detect of Vibrio parahaemolyticus Carrying trh Virulence Gene in Seafoods by Duplex PCR

作　　者：刘月苹 LIU Yueping(Tangshan Vocational Technical College,Tangshan 063004,China;School of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]唐山职业技术学院,河北唐山063004 [2]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《生物化工》2022年第1期21-25,共5页Biological Chemical Engineering

摘　　要：为建立有效鉴别副溶血性弧菌及携带耐热相关溶血毒素基因的毒性弧菌的方法,用副溶血性弧菌种属特异性基因tlh及毒性基因trh设计引物进行双重PCR检测,对双重PCR体系进行优化,并对其特异性及PCR灵敏度进行检测,验证双重PCR体系检测能力及对人工污染样品的灵敏度。结果表明,该方法对海产品中的副溶血性弧菌及携带trh毒性基因的弧菌具有检测特异性,且灵敏度较高,可为食品检测提供一定的参考依据。To establish an effective detection method for Vibrio parahaemolyticus and Vibrio parahaemolyticus carrying thermostable related hemolysin toxin gene(trh-virulence),primers targeting the Vibrio parahaemolyticus species specific gene tlh and virulent factor gene trh were designed,duplex PCR was used to identify the Vibrio parahaemolyticus as well as trh-virulence,including optimize the duplex PCR conditions,detect the specificity and sensitivity.The results show that the detection method is specific and sensitive,this method can specific and effective detect the Vibrio parahaemolyticus as well as trh-virulence,and provide valuable information for the food examination.

关 键 词：副溶血性弧菌 双重PCR 耐热相关溶血毒素基因 

分 类 号：R378.3[医药卫生—病原生物学]