A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection  

作　　者：Naixin Song Pengfei Xie Wen Shen Hanju Oh Yejia Zhang Flavia Vitale Mehdi Javanmard Mark G.Allen 

机构地区：[1]Department of Electrical and Systems Engineering,University of Pennsylvania,Philadelphia,PA,USA [2]Department of Electrical and Computer Engineering,Rutgers University,Piscataway,NJ,USA [3]Department of Physical Medicine and Rehabilitation,University of Pennsylvania,Philadelphia,PA,USA [4]Department of Orthopedic Surgery,University of Pennsylvania,Philadelphia,PA,USA [5]Corporal Michael J.Crescenz Veterans Affairs Medical Center,Philadelphia,PA,USA [6]Department of Bioengineering,School of Engineering and Applied Science,University of Pennsylvania,Philadelphia,PA,USA [7]Department of Neurology,Perelman School of Medicine,University of Pennsylvania,Philadelphia,PA,USA

出　　处：《Microsystems & Nanoengineering》2021年第6期139-150,共12页微系统与纳米工程（英文）

基　　金：This work was supported by DARPA Cooperative Agreement HR0011-16-2-0026 under the ElectRX program managed by Dr.E.Van Giesen;Fabrication was conducted at the Singh Center for Nanotechnology at the University of Pennsylvania,which is supported by the National Science Foundation NNCI2025608.

摘　　要：Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity,as well as rapid or real-time operation.Furthermore,microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation.Herein,we present microfabricated needle-shaped microwell impedance sensors for rapid-sample-to-answer,label-free detection of cytokines,and other biomarkers.The microneedle form factor allows sensors to be utilized in transcutaneous or transvascular sensing applications.In vitro,experimental characterization confirmed sensor specificity and sensitivity to multiple proteins of interest.Mechanical characterization demonstrated sufficient microneedle robustness for transcutaneous insertion,as well as preserved sensor function postinsertion.We further utilized these sensors to carry out real-time in vivo quantification of human interleukin 8(hIL8)concentration levels in the blood of transgenic mice that endogenously express hIL8.To assess sensor functionality,hIL8 concentration levels in serum samples from the same mice were quantified by ELISA.Excellent agreement between real-time in vivo sensor readings in blood and subsequent ELISA serum assays was observed over multiple transgenic mice expressing hIL8 concentrations from 62 pg/mL to 539 ng/mL.

关 键 词：NEEDLE utilized INSERTION 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]